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Q.How does a Nucleotide differ from a Nucleoside?

Jammu Kashmir JkboseJammu and Kashmir Board of School Education (Class 11) 2024Subjective· 2mImportance★★★★★
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Nucleosides lack a phosphate group; nucleotides are nucleosides with a phosphate group attached, and it is nucleotides (not nucleosides) that polymerize to form nucleic acids (DNA/RNA).

Step 1 — Building blocks.

Both DNA and RNA are polymers built from repeating units. Each unit has three chemical components:

  1. A nitrogenous base (purine: adenine, guanine; or pyrimidine: cytosine, thymine/uracil)
  2. A pentose sugar (deoxyribose in DNA, ribose in RNA)
  3. A phosphate group

Step 2 — Nucleoside.

When only the nitrogenous base is attached to the pentose sugar (via an N-glycosidic bond), without any phosphate group, the resulting molecule is called a nucleoside.

Example: adenosine (adenine + ribose), deoxyguanosine (guanine + deoxyribose).

Step 3 — Nucleotide.

When a phosphate group is further esterified to the sugar of a nucleoside (usually at the 5′-carbon), the resulting molecule is called a nucleotide.

Example: adenosine monophosphate (AMP), deoxyadenosine triphosphate (dATP).

Step 4 — Key difference and significance.

FeatureNucleosideNucleotide
CompositionBase + SugarBase + Sugar + Phosphate

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