Q.How does a Nucleotide differ from a Nucleoside?
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Start your 14-day free trial to unlock the full solution →Nucleosides lack a phosphate group; nucleotides are nucleosides with a phosphate group attached, and it is nucleotides (not nucleosides) that polymerize to form nucleic acids (DNA/RNA).
Step 1 — Building blocks.
Both DNA and RNA are polymers built from repeating units. Each unit has three chemical components:
- A nitrogenous base (purine: adenine, guanine; or pyrimidine: cytosine, thymine/uracil)
- A pentose sugar (deoxyribose in DNA, ribose in RNA)
- A phosphate group
Step 2 — Nucleoside.
When only the nitrogenous base is attached to the pentose sugar (via an N-glycosidic bond), without any phosphate group, the resulting molecule is called a nucleoside.
Example: adenosine (adenine + ribose), deoxyguanosine (guanine + deoxyribose).
Step 3 — Nucleotide.
When a phosphate group is further esterified to the sugar of a nucleoside (usually at the 5′-carbon), the resulting molecule is called a nucleotide.
Example: adenosine monophosphate (AMP), deoxyadenosine triphosphate (dATP).
Step 4 — Key difference and significance.
| Feature | Nucleoside | Nucleotide |
|---|---|---|
| Composition | Base + Sugar | Base + Sugar + Phosphate |
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