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Q.A double stranded DNA molecule has 20% of cytosine. Using Chargaff's law calculate the percentage of adenine in this DNA molecule.

Punjab PsebPSEB Punjab Class 12 Board 2019Subjective· 2mImportance★★★★★
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By Chargaff's rule %A = %T and %G = %C; with C = 20%, G is also 20%, leaving 60% for A+T, so A = 30%.

Chargaff's rule for double-stranded DNA states that the amount of adenine equals the amount of thymine, and the amount of guanine equals the amount of cytosine (because A pairs with T by two hydrogen bonds, and G pairs with C by three hydrogen bonds).

Given: %Cytosine (C) = 20%

Since %G = %C, %Guanine = 20%

So %G + %C = 20% + 20% = 40% …

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