Skip to content
← Biology

Biology · Class 12 Science

Punjab Pseb Class 12 Biology — Real Previous-Year Papers

with complete answers

Real previous-year board papers, year by year — the official exam pattern, the full question paper, and every question solved the concept-first way. Distinct from the chapter-wise textbook bank.

2018–2026
Years of papers
5
Total Papers
5
Real Board Papers
0
Sample papers
155
Real-paper Q & A
0
Sample-paper Q & A

Real board-paper questions available, by year

38 Q2026complete
38 Q2025complete
—2024Not available
—2023Not available
—2022Not available
—2021Not available
27 Q2020complete
26 Q2019complete
26 Q2018complete

PSEB Punjab Class 12 Board 2026 · Set ANNUAL

Real board examination

About this paper

The real Class-12 board examination held in 2026. Every question below is solved the concept-first way. Sample papers are labelled honestly — never shown as a past exam.

Total marks
70
Questions
38
Duration
180 min
Sections
4

The marks / questions / duration above are the official exam pattern. We currently have 38 of this paper’s questions (100% of the full paper), with 38 fully solved. Questions we couldn’t yet extract or verify are held — never shown as complete.

Sections & marks

SectionTypeQuestionsMarks eachTotal
ASection Acompulsory20120
BSection Bcompulsory10220
CSection Ccompulsory5315
DSection Dcompulsory3515
Total3870

The question paper

The questions we hold for this paper, laid out by section. Solutions are on the Answers tab.

Board Examination

Biology

PSEB Punjab Class 12 Board 2026 · Set ANNUAL

Series/Set: ANNUALRoll No. ________
Time Allowed: 3 hoursMaximum Marks: 70

General Instructions

  1. This question paper contains 38 questions divided into 4 sections — A, B, C, D.
  2. Section A comprises 20 questions of 1 mark each (compulsory).
  3. Section B comprises 10 questions of 2 marks each (compulsory).
  4. Section C comprises 5 questions of 3 marks each (compulsory).
  5. Section D comprises 3 questions of 5 marks each (compulsory).

Above is the official exam pattern. The questions printed below are those we currently hold for this paper.

Section A

compulsory · 1 mark each · 20 of 20 shown

Q1.
What causes the inactive form of Bt toxin (Protoxin) to get converted into it's active form in the body of an insect?
  • (a) Temperature of the gut
  • (b) Enzymes present in saliva
  • (c) Alkaline pH of the gut
  • (d) There is no specific reason
[1]
Q2.
Two species competing for the same resource can avoid competition by choosing different habits. This phenomenon is called _________ and was supported by _________.
  • (a) Competitive exclusion, Gause
  • (b) Competitive exclusion, MacArthur
  • (c) Resource partitioning, Gause
  • (d) Resource partitioning, MacArthur
[1]
Q3.
The flow of energy through lower to higher trophic levels in an ecosystem is :
  • (a) always unidirectional.
  • (b) sometimes unidirectional and sometimes bidirectional.
  • (c) always bidirectional.
  • (d) cannot be determined with certainty.
[1]
Q4.
Meenu visited Mahendra Chaudhary Zoolgical Park (Chattbir Zoo) as part of her environmental studies school tour. She observed several animals such as tigers, rhinoceroses and lions being maintained and bred in enclosures. This is an example of which type of conservation?
  • (a) In situ conservation
  • (b) Ex situ conservation
  • (c) On-site conservation
  • (d) Natural habitat conservation
[1]
Q5.
Geitonogamy involves transfer of pollen grains :
  • (a) from anther to stigma of the same flower.
  • (b) from anther to stigma of another flower on the same plant.
  • (c) from anther to stigma of a flower on a different plant of the same species.
  • (d) between flowers of different species.
[1]
Page 1 of 6
Q6.
The testes in humans are located outside the abdominal cavity within a pouch called the scrotum. This positioning helps in : (a) avoiding compression by internal organs. (b) maintaining a temperature slightly lower than the body temperature for sperm formation. (c) protecting the testes from physical injury during movement. (d) helping in the storage of urine before excretion.
[1]
Q7.
Diaphragms are contraceptive devices used by females. Which of the following statement is correct about diaphragm? (a) They are introduced into the uterus to prevent implantation. (b) They are placed to cover the cervical region and act as a physical barrier for sperm entry. (c) They act as spermicidal agents. (d) They prevent ovulation by hormonal regulation.
[1]
Q8.
A pure tall (TT) plant is crossed with a pure dwarf (tt) plant to produce F1 offspring. The F1 offspring are then self-crossed. What is the ratio of true-breeding tall (TT) to true-breeding dwarf (tt) plants in the F2 generation? (a) 1 : 1 (b) 1 : 2 : 1 (c) 1 : 3 (d) 3 : 1
[1]
Q9.
In E.coli, the lac operon gets switched on when : (a) lactose is present and it binds to the repressor. (b) repressor binds to operator. (c) RNA polymerase binds to the operator. (d) lactose is absent.
[1]
Q10.
Darwin observed different types of beaks in finches adapted to different feeding habits on the Galapagos Islands. This diversity provides evidence for : (a) intraspecific competition. (b) interspecific competition. (c) origin of species by natural selection. (d) origin of species by mutation.
[1]
Q11.
Identify the parts labelled A, B, C and D in the diagram of an antibody molecule and select the correct options : (a) A – Light chains, B – Heavy chains, C – Antigen-binding sites, D – Disulphide bonds (b) A – Disulphide bonds, B – Antigen-binding sites, C – Heavy chains, D – Light chains (c) A – Antigen-binding sites, B – Light chains, C – Heavy chains, D – Disulphide bonds (d) A – Antigen-binding sites, B – Disulphide bonds, C – Light chains, D – Heavy chains
[1]
Q12.
The enzyme Taq polymerase used in PCR has been isolated from the bacterium : (a) Agrobacterium tumefaciens (b) Thermus aquaticus (c) Streptomyces albus (d) Escherichia coli
[1]
Q13.
Alexander Fleming, Ernst Chain and Howard Florey were awarded the Nobel Prize in 1945 for the discovery of _____.
[1]
Q14.
The sequence of base pairs in a double-stranded DNA or RNA molecule that reads the same in forwards and backward on each strand is called _____ nucleotide sequence.
[1]
Page 2 of 6
Q15.
The process of release of ovum from the mature follicle is called _____.
[1]
Q16.
_____ test is performed to confirm typhoid.
[1]
Q17.
Write True or False. Saccharomyces cerevisiae is used in the production of alcohol and bread.
[1]
Q18.
Write True or False. The Amazon rainforest is known as the "lungs of the planet" because it produces a large amount of carbon dioxide.
[1]
Q19.
Write True or False. Surgical methods of contraception prevent gamete formation.
[1]
Q20.
Write True or False. Cocaine is obtained from the leaves of the Erythroxylum coca and acts as a stimulant on the central nervous system.
[1]
Section B

compulsory · 2 marks each · 10 of 10 shown

Q1.
Samina saw her mother adding a little curd to warm milk at night. She asked why she was doing this? She replied that the curd acts as an inoculum to convert milk into curd. (a) Name the bacterium responsible. (b) Explain how adding a small amount of curd (inoculum) helps in making the entire milk turn into curd?
[2]
Q2.
What is biolistic method for introducing alien DNA into a host cell?
[2]
Q3.
Bt toxin is produced by bacteria Bacillus thuringiensis but it does not kill the bacteria itself. Why is it so?
[2]
Page 3 of 6
Q4.
Define food chain. Construct a grazing food chain using any 5 of the following : Grass, Earthworm, Snake, Vulture, Frog, Grasshopper, Decaying plant matter.
[2]
Q5.
Draw a well-labelled diagram of a longitudinal section of a flower showing all its parts.
[2]
Q6.
State the role of oxytocin in parturition.
[2]
Q7.
In case of an infertile couple, the male has very low sperm count but the female has normal functioning reproductive system. Suggest and explain the Assisted Reproductive Technology (ART) useful to this couple.
[2]
Q8.
A man with blood group A marries a woman with blood group B. They have two children, one with blood group AB and the other with blood group O. Work out a genetic cross to show how this is possible?
[2]
Q9.
Classify the following pairs as analogous or homologous organs : • Wings of butterflies and birds • Thorns of bougainvillea and tendrils of cucurbita • Forelimbs of whale, bat, cheetah and human • Sweet potato and Potato
[2]
Q10.
Write any two points of difference between innate and acquired immunity.
[2]
Page 4 of 6
Section C

compulsory · 3 marks each · 5 of 5 shown

Q1.
The diagram below shows an agarose gel electrophoresis setup : (a) Label the positive (+) and negative (−) terminals. (b) What type of charge is carried by DNA molecules and how does this property help in their separation? (c) Name the method used to isolate the separated DNA fragments from the gel.
[3]
Q2.
Name the type of interaction seen in each of the following examples : (a) Cuscuta growing on a hedge plant (b) Mycorrhizae living on the roots of higher plants (c) An orchid growing on the branch of a mango tree
[3]
Q3.
Given diagram is of transcription unit. Identify the strands A and B in this diagram and write the basis on which you identified them.
[3]
Q4.
After industrialisation in England, the population of melanised moths (dark-coloured) increased while that of white-winged moths decreased. (a) State the reason behind this change. (b) Explain this change in terms of natural selection.
[3]
Q5.
Name the causing agent of malaria. Also write down its symptoms and modes of transmission.
[3]
Page 5 of 6
Section D

compulsory · 5 marks each · 3 of 3 shown

Q1.
Read the paragraph carefully and answer the following questions : A constant input of solar energy is the basic requirement for any ecosystem to function and sustain. Primary production is defined as the amount of biomass or organic matter produced per unit area over a time period by plants during photosynthesis. It is expressed in terms of weight (gm⁻²) or energy (kcal m⁻²). The rate of biomass production is called productivity. It is expressed in terms of gm⁻² yr⁻¹ or (kcal m⁻²) yr⁻¹ to compare the productivity of different ecosystems. It can be divided into gross primary productivity (GPP) and net primary productivity (NPP). Gross primary productivity of an ecosystem is the rate of production of organic matter during photosynthesis. A considerable amount of GPP is utilised by plants in respiration. Gross primary productivity minus respiration losses (R), is the net primary productivity (NPP). Net primary productivity is the available biomass for the consumption to heterotrophs (herbivores and decomposers). Secondary productivity is defined as the rate of formation of new organic matter by consumers. Primary productivity depends on the plant species inhabiting a particular area. It also depends on a variety of environmental factors, availability of nutrients and photosynthetic capacity of plants. Therefore, it varies in different types of ecosystems. (a) Name the factors that influence the primary productivity of an ecosystem. [1] (b) Suppose the GPP of a grassland ecosystem is 2000 kcal m⁻² yr⁻¹ and NPP is 1200 kcal m⁻² yr⁻¹. Calculate the respiratory loss (R). [1] (c) Why is all the primary productivity not available to herbivores for consumption? [1] (d) Differentiate between GPP and NPP. [2]
[5]
Q2.
(a) What is meant by monosporic development of the female gametophyte? [2] (b) Draw a diagram of a mature embryo sac and label any four parts. [3]
[5]
Q3.
Given below is a karyotype obtained after analysis of foetal cells : (a) Based on the karyotype, what chromosomal disorder is detected? [1] (b) Write its cause. [2] (c) Write the symptoms of the disorder. [2]
[5]
Page 6 of 6