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Q.Which part of the bacterial cell is targeted in gram staining?

Telangana TsbieTelangana Board of Intermediate Education (Intermediate 1st Year) 2026Subjective· 2mImportance★★★★★
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Gram staining targets the bacterial cell wall (its peptidoglycan layer), which determines Gram-positive vs Gram-negative reaction.

Gram staining is a differential staining technique used to classify bacteria into two groups. The part of the bacterial cell that is responsible for the staining reaction is the cell wall, in particular its peptidoglycan (murein) layer.

  • Gram-positive bacteria have a thick peptidoglycan layer that retains the primary stain (crystal violet-iodine complex) and appear violet/purple. …

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