Q.Describe the experiment carried out by Hershey and Chase. Write the conclusion they arrived at.
🔒You're viewing a preview — the full solution, concept, methods & PYQ mapping are locked.
🔒 Start your 14-day free trial to unlock the full solution →Part (a)Concept understanding — Nucleic Acid Functions
Nucleic Acid Functions – A First Look
Think of a living organism as a giant, incredibly complex factory. Every second, this factory needs to produce thousands of different products—proteins, enzymes, hormones, structural materials—in exactly the right amounts, at exactly the right places, and at exactly the right times. How does the factory know what to build and when? It needs a master blueprint and a set of working copies that can be carried to the assembly lines.
That master blueprint is DNA (deoxyribonucleic acid). The working copies are RNA (ribonucleic acid). Together, they are the nucleic acids, and their job is to store, transmit, and execute the genetic information that makes every living thing what it is.
The Two Main Functions
1. DNA – The Permanent Blueprint (Storage and Inheritance)
DNA is the long-term, stable repository of genetic information. It is like the original architectural plan for the entire factory, locked in a secure vault. Its functions are:
- Storing genetic information: DNA contains the instructions for building every protein the organism will ever need. These instructions are written in a chemical language using four "letters" (nucleotides: A, T, G, C). The sequence of these letters is the code.
- Replication (making copies): Before a cell divides, DNA makes an exact copy of itself. This ensures that each daughter cell receives a complete set of instructions. This is why children inherit traits from their parents—the DNA blueprint is passed down.
- Transmission to offspring: DNA is the molecule of heredity. It is passed from parents to offspring, carrying the genetic information that determines everything from eye colour to susceptibility to certain diseases.
DNA never leaves the nucleus of a cell. It is too precious and too large to move around. It stays safely inside, like a reference book that cannot be taken out of the library.
2. RNA – The Working Copy (Execution of the Blueprint)
RNA is the temporary, mobile copy of specific parts of the DNA blueprint. It is like a photocopy of a single page from the master plan, which a worker can carry to the factory floor. Its functions are:
- Transcription (copying the message): A specific segment of DNA (a gene) is used as a template to make a complementary RNA molecule. This RNA copy is called messenger RNA (mRNA).
- Translation (reading the message to build a protein): The mRNA travels out of the nucleus to the ribosomes (the protein-building machines). Here, another type of RNA called transfer RNA (tRNA) reads the mRNA code and brings the correct amino acids, one by one, to build a protein chain.
- Catalysis (as a biological catalyst): Some RNA molecules, called ribozymes, can act as enzymes and speed up chemical reactions. This is a less well-known but crucial function, especially in the ribosome itself (which is partly made of RNA).
Why This Matters for You
Even if you never touch a test tube, understanding nucleic acid functions helps you grasp:
- Why children resemble their parents: DNA is the hereditary material.
- How vaccines work: Many vaccines use mRNA to instruct your cells to produce a harmless piece of a virus, training your immune system.
- How genetic disorders arise: A mistake in the DNA sequence (a mutation) can lead to a faulty protein, causing diseases like sickle cell anaemia or cystic fibrosis. …
Part (b)Concept understanding — Darwinian Natural Selection
Imagine you walk into a crowded room. Some people are naturally louder, some are quieter. Some are taller, some shorter. Now imagine that, for some reason, the room is suddenly plunged into darkness, and everyone has to find the exit by touch alone. Who do you think will get out first? Probably not the loudest talker, but the person who happens to have the most sensitive fingertips or the best memory of where the door was.
That simple scenario captures the core of Darwinian natural selection. It is not about being "better" in some moral or absolute sense. It is about being a better fit for the specific situation you are in.
The Everyday Intuition: "Survival of the Fittest" — But What Does "Fittest" Mean?
The phrase "survival of the fittest" is often misunderstood. It does not mean the strongest, fastest, or most aggressive individual wins. In biology, "fitness" has a very specific meaning: the ability to survive long enough to reproduce and leave offspring.
Think of it this way: in a game of musical chairs, the "fittest" player isn't the one who dances the best. It's the one who, when the music stops, is standing on a chair. The chair is the environment. The player's ability to grab that chair is their fitness.
Darwin never used the phrase "survival of the fittest" in his first edition of On the Origin of Species. It was coined by Herbert Spencer, a philosopher, and Darwin adopted it in later editions. Darwin's own term was "natural selection," which is a more accurate description of the process.
The Precise Meaning: How Natural Selection Works
Natural selection is not a conscious force. It is a blind, automatic process that follows from three simple facts that are always true in any population of living things:
-
Variation: Individuals in a population are not identical. Even within a species, there is a range of traits — different beak sizes in birds, different fur colours in rabbits, different heights in humans. This variation is the raw material.
-
Inheritance: Many of these variations are passed from parents to offspring. A tall parent is more likely to have tall children. A fast-running cheetah is more likely to have fast-running cubs.
-
Differential Survival and Reproduction: More individuals are born than can possibly survive. Resources (food, water, shelter, mates) are limited. This creates a "struggle for existence." Individuals with traits that give them even a slight advantage in this struggle are more likely to survive and, crucially, to reproduce.
The result: Over many generations, the traits that helped those individuals survive and reproduce become more common in the population. The traits that were less helpful become rarer. The population gradually changes to become better suited to its environment.
Natural selection does not create new traits. It only acts on the variation that already exists. It is like a sieve, not a sculptor. The sieve lets through the grains of sand that are the right size for the hole, and holds back the rest. The environment is the sieve.
Why It Matters: The Engine of Adaptation
Natural selection is the mechanism that explains how life becomes adapted to its surroundings. It is why:
- A cactus has spines instead of leaves (to reduce water loss in a desert).
- A polar bear has white fur (to blend in with snow and ice).
- A human has an opposable thumb (to grip tools).
It is not a plan or a goal. It is a consequence of simple, observable facts playing out over vast stretches of time. The NCERT textbook for Class 12 Biology (Chapter 6, "Evolution") states this clearly: "Natural selection is a process in which heritable variations enabling better survival are enabled to reproduce and leave greater number of progeny."
A Few Key Points to Remember
- It acts on individuals, but it changes populations. An individual does not evolve. The population as a whole changes over generations. …
Part (a)
Hershey and Chase infected E. coli with bacteriophages whose DNA was labelled with 32P and whose protein coat was labelled with 35S. After blending and centrifuging, the radioactive 32P (DNA) was found inside the bacteria while the 35S (protein) stayed outside. …
Part (a): Hershey and Chase labelled phage DNA with 32P and protein with 35S; only DNA entered the bacterium, proving DNA is the genetic material.
Part (b): Peppered-moth colour change (industrial melanism) and resistance to herbicides/pesticides/antibiotics are both natural selection — a favourable variant increases under selective pressure.
Part (a)
Hershey and Chase (1952) used bacteriophages to settle whether DNA or protein carries heredity. DNA has phosphorus but no sulphur; protein has sulphur but no phosphorus.
- Phages grown on 32P had radioactive DNA.
- Phages grown on 35S had radioactive protein coats.
The labelled phages were allowed to attach to and infect E. coli. The mixture was agitated in a blender to detach the phage coats and then centrifuged. The heavier bacterial cells settled as a pellet.
Result: the 32P (DNA) was recovered with the bacterial pellet — it had entered the cells — while the 35S (protein) remained in the supernatant, outside the cells. …
- AP EAPCET 2026Set ap-2026-05-20-FN1 markMCQQ.Darwin called the macro-variations as (A) Discontinuous variation (B) Chromosomal mutations (C) Gene mutations (D) Sports of nature
›Reveal solutionSolution
Darwin's own term for large, discontinuous (macro-) variations was 'sports of nature'; De Vries later renamed this phenomenon 'mutation.'
Concept and Intuition
Darwin's theory of evolution by natural selection primarily relied on small, continuous variations accumulating gradually across generations. However, he also observed occasional large, abrupt changes appearing in a single generation, distinct from ordinary fluctuating variation — he called these unusual, discontinuous jumps 'sports.' This idea anticipated the later, more rigorous concept of mutation developed by Hugo de Vries from his breeding experiments on the evening primrose (Oenothera lamarckiana).
Step-by-Step Solution
- Recall the two categories of variation Darwin distinguished: gradual, continuous ('individual differences') vs sudden, large, discontinuous changes. …
- AP EAPCET 2025Set ap-2025-05-20-AN1 markMCQQ.Assertion (A) : In the polluted area, Birmingham, black peppered moths were abundant. In the non polluted area, Dorset, grey forms were abundant. Reason (R) : Natural selection. Identify the correct option from the following (A) (A) and (R) are true. (R) is correct explanation for (A) (B) (A) and (R) are true. But (R) is not the correct explanation for (A) (C) (A) is true, but (R) is false (D) (A) is false, but (R) is true
›Reveal solutionSolution
The classic peppered moth (industrial melanism) example shows dark moths thriving in
soot-polluted Birmingham and pale moths thriving in unpolluted Dorset — a textbook case
of natural selection acting through differential camouflage/predation, so both
statements are true and the Reason correctly explains the Assertion. Answer: (A).
Concept and Intuition
The peppered moth (Biston betularia) is the most famous illustration of natural
selection in real time. Before industrialisation, pale, lichen-camouflaged moths were
favoured because they blended into lichen-covered tree bark, escaping bird predation,
while dark (melanic) moths stood out and were eaten more often. As industrial pollution
in areas like Birmingham blackened tree trunks with soot (killing the lichen), the
camouflage advantage reversed: dark moths now blended in better and pale moths became
conspicuous. In unpolluted regions like Dorset, tree bark remained lichen-covered, so
pale moths retained their camouflage advantage there. This differential,
environment-dependent survival and reproduction is a direct demonstration of natural
selection.
Step-by-Step Solution
- Assertion: in polluted Birmingham, black moths were abundant; in unpolluted Dorset, grey (pale) moths were abundant — this matches the well-documented historical/field observations. True.
- Reason: natural selection — the differential predation based on camouflage against …
- AP EAPCET 2024Set ap-2024-05-16-FN1 markMCQQ.Match the following: List-1: A. Directional selection, B. Genetic load, C. Bottle neck effect, D. Centrifugal selection List-2: I. Polydactylic dwarfs, II. Darwin's finches, III. DDT resistant mosquitoes, IV. Tailed human baby, V. Sickle cell anaemia (A) A-III, B-IV, C-I, D-V (B) A-II, B-IV, C-V, D-I (C) A-III, B-V, C-I, D-II (D) A-III, B-IV, C-I, D-II
›Reveal solutionSolution
Four classic evolution examples map onto four selection/drift concepts: directional (DDT resistance), genetic load (sickle-cell balanced polymorphism), bottleneck/founder effect (Amish polydactylic dwarfism), and centrifugal/disruptive selection (Darwin's finches).
Concept and Intuition
- Directional selection favours one phenotypic extreme over the population mean — DDT-resistant mosquitoes are a textbook case of a population shifting toward the resistant extreme under selection pressure.
- Genetic load refers to the reduced average fitness of a population due to deleterious alleles maintained in the gene pool, often via heterozygote advantage — the sickle-cell allele persisting in malaria-endemic regions (heterozygotes resist malaria, but homozygotes suffer disease) is the classic example.
- Bottleneck/founder effect occurs when a population's gene pool is drastically reduced; the Old Order Amish community, descended from a small founder population, shows an unusually high frequency of polydactyly combined with dwarfism (Ellis–van Creveld syndrome).
- Centrifugal (disruptive) selection favours both phenotypic extremes over the intermediate form — Darwin's finches on the Galápagos show bimodal beak-size selection tied to differing food resources.
Step-by-Step Solution …
- AP EAPCET 2024Set ap-2024-05-17-AN1 markMCQQ.The driving force of evolution is (A) Reproductive isolation (B) Natural selection (C) Artificial selection (D) Mutations
›Reveal solutionSolution
This tests foundational evolutionary theory; natural selection is the primary driving force of evolution.
Concept and Intuition
Evolution requires raw material (heritable variation, arising from mutation and recombination) and a mechanism that channels that variation into adaptive change. While mutation supplies variation and reproductive isolation can lead to speciation, the actual directional "engine" that shapes populations by favouring some variants over others across generations is natural selection — Darwin's central mechanism of evolution.
Step-by-Step Solution
- Reproductive isolation (A) is a consequence/mechanism that leads to speciation, not the fundamental driving force of evolutionary change itself.
- Artificial selection (C) is a human-directed analogue of natural selection, used to illustrate the concept, but it is not the driving force operating in nature. …
- AP EAPCET 2024Set ap-2024-05-17-AN1 markMCQQ.Select the correct statement: (A) Hardy-Weinberg law is applicable to small populations. (B) Sewall Wright effect is applicable to large populations. (C) Development of resistance to DDT by mosquitoes is an example for directional selection. (D) If one species diverges to become two or more species, it is called anagenesis.
›Reveal solutionSolution
This tests population genetics concepts; DDT resistance in mosquitoes is the standard textbook example of directional selection.
Concept and Intuition
Several statements here test precise definitions in population genetics and evolution:
- The Hardy-Weinberg law describes allele frequency equilibrium and strictly applies to LARGE, randomly mating populations free of selection, mutation, migration, and drift.
- The Sewall Wright effect (genetic drift) is the random change in allele frequencies due to chance, and its effects are most pronounced in SMALL populations.
- Directional selection shifts a population's phenotype distribution consistently in one direction in response to an environmental pressure — DDT exposure selecting for resistant mosquitoes is the textbook example.
- Anagenesis refers to evolutionary change WITHIN a single lineage without splitting into new species; the SPLITTING of one species into two or more is called cladogenesis, not anagenesis.
Step-by-Step Solution
- (A) is wrong: Hardy-Weinberg applies to large populations, not small ones.
- (B) is wrong: the Sewall Wright effect (genetic drift) applies to small populations, not large ones — exactly the reverse of what's stated. …
- AP EAPCET 2024Set ap-2024-05-17-FN1 markMCQQ.In DNA, bond between the phosphate and hydroxyl group of sugar (A) Hydrogen bond (B) Glycosidic bond (C) Ester bond (D) Peptide bond
›Reveal solutionSolution
The single linkage joining a phosphate group to a sugar's –OH in the nucleic-acid backbone is a phosphoester (ester) bond; two of these make the phosphodiester bridge between adjacent sugars.
Concept and Intuition
A nucleotide is built from a nitrogenous base, a pentose sugar, and a phosphate group. The phosphate is attached to the sugar via a condensation reaction between the phosphate group and a hydroxyl (–OH) group on the sugar, releasing water — this is chemically an esterification, so the resulting bond is an ester (phosphoester) bond. In the DNA/RNA backbone, the same phosphate bridges the 3′-OH of one sugar and the 5′-OH of the next sugar via two such ester bonds, together called a phosphodiester bond.
Step-by-Step Solution
- Identify the two groups reacting: a phosphate group (–PO₄) and a sugar hydroxyl (–OH).
- Recognize that phosphate + hydroxyl → phosphate ester + water is the classic definition of an esterification reaction. …
🎓Unlock everything free for 14 days
- ✓Full step-by-step solutions
- ✓Concept-first explanations
- ✓Methods, shortcuts & mistakes
- ✓PYQ mapping + timed mock tests
Full access for 14 days. No credit card required.