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NCERT Exemplar · Q14

Q.Which one of the following is not a nitrogen-fixing organism?

(a) Anabaena
(b) Nostoc
(c) Azotobacter
(d) Pseudomonas
Chhattisgarh CgbseMCQ· 1mImportance★★★★★
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Among the given options, Pseudomonas is the only one that is not a nitrogen-fixing organism — the other three are well-known nitrogen fixers.

Nitrogen fixation is the process by which atmospheric nitrogen (N2) is converted into ammonia (NH3), a form that living organisms can use to build proteins and nucleic acids. Only certain prokaryotes — bacteria and cyanobacteria — possess the enzyme nitrogenase that makes this possible. In the NCERT Class 11 Biology textbook (Chapter 12, Mineral Nutrition), three main groups of nitrogen-fixing organisms are discussed: free-living bacteria, symbiotic bacteria (like Rhizobium), and cyanobacteria.

Let’s look at each option:

  • Anabaena and Nostoc are both cyanobacteria (blue-green algae). They are free-living nitrogen fixers, and Anabaena is also famous for its symbiotic association with the water fern Azolla, which is used as a biofertilizer in rice paddies. NCERT explicitly mentions both as nitrogen-fixing cyanobacteria.

  • Azotobacter is a free-living, aerobic bacterium that fixes nitrogen in the soil. It is also listed in the NCERT as a major free-living nitrogen fixer.

  • Pseudomonas is a genus of bacteria that is incredibly versatile — some species are decomposers, some are plant pathogens, and others are used in bioremediation. However, no species of Pseudomonas is known to fix nitrogen. NCERT does not include it among nitrogen-fixing organisms, and it is not mentioned in that context anywhere in the syllabus. …

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