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NCERT Exemplar · Q8

Q.The principle involved in paper chromatography is

(i) Adsorption
(ii) Partition
(iii) Solubility
(iv) Volatility
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Paper chromatography separates mixtures based on partition of components between a stationary liquid phase (water held in paper) and a moving solvent. The correct principle is partition, not adsorption.

Paper chromatography is a technique used to separate and identify components of a mixture — like pigments in ink or plant dyes. To understand why it works, you need to think about what happens at the molecular level when the solvent creeps up the paper.

The paper itself is not the stationary phase. It acts as a support for a thin layer of water that gets trapped in its cellulose fibres. This water is the real stationary phase. The solvent (mobile phase) moves up the paper by capillary action, carrying the mixture's components along. As the solvent travels, each component repeatedly partitions (distributes itself) between the stationary water layer and the moving solvent. Components that prefer water (more polar) spend more time in the stationary phase and move slowly; components that prefer the solvent (less polar) move faster. This difference in partition coefficients is what causes separation.

Now let's examine each option:

  1. Adsorption — This is the principle behind thin-layer chromatography (TLC) and column chromatography, where the stationary phase is a solid (like silica gel) that actively binds components to its surface. In paper chromatography, the stationary phase is a liquid (water), not a solid. The components dissolve into the water layer rather than sticking to the paper's surface. So adsorption is not the primary principle here.

  2. Partition — This is exactly what happens. Each solute molecule distributes itself between two immiscible liquids: the water held in the paper and the organic solvent moving up. The ratio of concentrations in these two phases (the partition coefficient) determines how far the component travels. This is the correct principle. …

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