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Q.Describe Hatch and Slack Pathway. OR Describe TCA (Tri Carboxylic Acid) cycle.

Haryana BsehBoard of School Education Haryana (Senior Secondary Part-I / Class 11) 2021Subjective· 5mImportance★★★★★
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C4 plants fix CO2 twice — once in mesophyll cells (as a 4-carbon acid) and again in bundle-sheath cells (via the Calvin cycle) — to concentrate CO2 and minimise photorespiration.

The Hatch and Slack pathway (C4 pathway) operates in C4 plants (e.g. maize, sugarcane, sorghum) that are adapted to hot, dry, high-light environments and show a distinctive leaf anatomy called Kranz anatomy, where bundle sheath cells (containing chloroplasts, arranged in a ring around the vascular bundle) surround the mesophyll cells.

  1. Primary CO2 fixation (in mesophyll cells): atmospheric CO2 is first fixed by the enzyme PEP carboxylase (PEPcase), which has a very high affinity for CO2, onto the 3-carbon acceptor phosphoenolpyruvate (PEP), forming the 4-carbon compound oxaloacetic acid (OAA) — this is why the pathway is called the C4 pathway.
  2. OAA is quickly converted into another 4-carbon acid, usually malic acid (malate) or aspartic acid.
  3. This C4 acid is then transported from the mesophyll cells into the adjoining bundle sheath cells.
  4. In the bundle sheath cells, the C4 acid is broken down (decarboxylated) to release CO2 and pyruvate (a 3-carbon compound).
  5. The released CO2 now enters the standard Calvin cycle (C3 cycle) operating within the bundle sheath chloroplasts, where it is fixed by RuBisCO in the usual way to make sugars. …

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