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NCERT Exemplar · Q19

Q.What is the cross between the progeny of F1 and the homozygous recessive parent called? How is it useful?

Meghalaya MboseShort· 2mImportance★★★★★
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The cross between the F1 progeny and its homozygous recessive parent is called a Test Cross, and it is used to determine the unknown genotype of an individual exhibiting a dominant phenotype.

Gregor Mendel's pioneering work with garden peas established the fundamental principles of heredity. He observed that when two true-breeding parents differing in a single trait were crossed, the first generation of offspring, known as the First Filial (F1) generation, uniformly expressed only one of the parental traits. This expressed trait was termed dominant, while the unexpressed one was recessive. For example, crossing a true-breeding tall pea plant with a true-breeding dwarf pea plant resulted in all tall F1 progeny, indicating tallness is dominant over dwarfness.

However, these F1 tall plants were not genetically identical to the true-breeding tall parent. While both appeared tall, the true-breeding parent carried two alleles for tallness (homozygous dominant), whereas the F1 progeny carried one allele for tallness and one for dwarfness (heterozygous). To distinguish between an individual that is homozygous dominant and one that is heterozygous, both of which display the dominant phenotype, a specific type of cross is employed.

The cross between the F1 progeny (or any individual showing a dominant phenotype) and the homozygous recessive parent is known as a Test Cross.

Note

The homozygous recessive parent is always chosen for a test cross because it can only contribute recessive alleles to its offspring. This ensures that any dominant alleles observed in the offspring must have come from the individual whose genotype is being tested, making the results clear and unambiguous.

The primary utility of a test cross lies in its ability to determine the unknown genotype of an individual that expresses a dominant phenotype. An individual showing a dominant trait could either be homozygous dominant (e.g., TT for tallness) or heterozygous (e.g., Tt for tallness). A test cross helps differentiate between these two possibilities.

Consider an example where we have a pea plant producing yellow seeds, and we know that yellow seed colour is dominant over green seed colour. We want to determine if this yellow-seeded plant is homozygous dominant (YY) or heterozygous (Yy). We would cross this unknown yellow-seeded plant with a homozygous recessive green-seeded plant (yy).

There are two distinct outcomes for the progeny of this test cross, each revealing the genotype of the unknown parent:

  • If the unknown dominant individual is homozygous dominant (YY):

    When a YY plant is crossed with a yy plant, all the offspring will inherit a 'Y' allele from the first parent and a 'y' allele from the second, resulting in all heterozygous (Yy) progeny. Consequently, all the offspring will display the dominant phenotype, meaning all will produce yellow seeds.

  • If the unknown dominant individual is heterozygous (Yy): …

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