Q.A cell has 32 chromosomes. It undergoes mitotic division. What will be the chromosome number (N) during metaphase? What would be the DNA content (C) during anaphase?

Anaphase Chromatid Separation

Imagine you are holding a long, stretched-out spring between your two hands. If you cut it exactly at the middle, each hand now holds one half. That is the core picture of anaphase — but with a crucial twist: the "cut" happens only when the cell is ready, and the two halves are actively pulled apart, not just released.

The Intuition: Why Separate at All?

A cell has spent hours duplicating every chromosome into two identical copies (sister chromatids) held together at a central region called the centromere. These copies are like a pair of identical twins holding hands. For the cell to divide into two daughter cells, each new cell must get one complete set of genetic instructions. The twins must let go and walk to opposite ends of the cell. That letting-go and walking is anaphase.

The separation is not passive. The cell does not simply dissolve the glue and let the chromatids drift. It actively reels them in, like a fisherman pulling a line, ensuring each daughter cell gets exactly one copy of every chromosome.

The Precise Statement

Anaphase chromatid separation is the simultaneous splitting of the centromere that holds sister chromatids together, followed by the poleward movement of each daughter chromatid along shortening spindle fibres attached to its kinetochore.

The Two Movements in Detail

Anaphase is often split into two concurrent processes:

Anaphase A — Chromatids move to the poles. The spindle fibres (microtubules) attached to the kinetochore at the centromere shorten by losing tubulin subunits at the kinetochore end. The chromatid is literally reeled in, like a rope being pulled from the pole. The kinetochore "walks" along the shortening microtubule.

Anaphase B — The poles themselves move apart. The spindle fibres between the two poles (interpolar microtubules) elongate, pushing the poles farther from each other. This stretches the cell and increases the separation between the two sets of chromatids.

Both movements happen simultaneously in most cells. The result is that by the end of anaphase, each pole has a complete set of chromosomes, and the cell is ready to divide its cytoplasm (cytokinesis).

The Molecular Trigger …