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Q.You can easily study pollen germination by dusting some pollen from flowers such as pea, chickpea, Crotalaria, balsam, and Vinca on a glass slide containing a drop of sugar solution (about 10 per cent). After about 15–30 minutes, observe the slide under the low power lens of the microscope. What do you expect to see?

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✓ Free question

On a 10% sugar-solution slide, viable pollen grains germinate within 15-30 minutes and put out visible pollen tubes — this practical directly demonstrates the first step of pollen-pistil interaction (§1.2.3) outside the flower.

Why this works: a sugar solution approximates the sucrose-rich medium the stigma surface naturally provides, which stimulates a compatible, viable pollen grain to germinate — the same trigger that starts pollen-tube growth in vivo after pollination.

What you should observe under the low-power lens:

  1. Most pollen grains will appear as small, roughly spherical bodies on the slide (their genus-specific exine ornamentation may be visible).
  2. From a proportion of the grains, a thin pollen tube emerges through one of the grain's germ pores and grows outward into the sugar solution.
  3. The pollen tube carries the grain's two male gametes (the vegetative cell guiding the tube's growth, with the generative cell/its two sperm cells inside it) — exactly as it would when growing through the style toward the micropyle of an ovule in a real pollinated flower.

This is why the activity is a useful, simple classroom proxy for pollen-pistil interaction: it isolates and makes directly visible the germination + tube-growth step that is otherwise hidden inside the pistil.

✓Final answer

Pollen tubes growing out of the germinated pollen grains — visible proof that the pollen was viable and has begun the same tube-growth process it would use inside a real pistil.

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