Biology · Ch 8 — Improvement in Food Production
Micropropagation
Micropropagation
Micropropagation is the practical application of totipotency to solve a very ordinary problem: how to multiply a single superior plant -- one that combines high yield, disease resistance and good quality, whether bred conventionally or simply an outstanding individual found in a farmer's field -- into thousands of identical copies quickly, without waiting for it to set seed and without the genetic reshuffling that sexual reproduction would introduce.
How it works
A small explant taken from the chosen parent plant (commonly a shoot tip or an axillary bud, since these already contain actively dividing meristematic tissue) is established on a sterile nutrient medium. Under the right balance of growth regulators, the explant proliferates into a mass of new shoots or an undifferentiated callus; individual shoots, or plantlets regenerated from the callus, are then repeatedly sub-cultured onto fresh medium, multiplying the original explant's genetic material many-fold at each cycle, before finally being rooted and gradually acclimatised (hardened off) to normal soil and atmospheric conditions outside the sterile culture vessel.
Why it matters …
What this figure shows. A five-panel labelled flow diagram showing the sequence of stages in micropropagation, left to right. Panel 1 shows a shoot tip or axillary bud being excised from a chosen elite parent plant, labelled 'explant collection'. Panel 2 shows this explant placed on a sterile nutrient medium in a culture vessel, labelled 'culture establishment'. Panel 3 shows the same explant having proliferated into a cluster of several small new shoots, labelled 'shoot multiplication (repeated sub-culturing)', with a small looping arrow indicating this stage is repeated over several cycles to build up large numbers. Panel 4 shows an individual small shoot with a newly formed root system in a fresh culture vessel, labelled 'rooting'. Panel 5 shows a young potted plantlet growing in ordinary soil under a clear humidity dome, labelled 'hardening / acclimati …