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Physics · Ch 9 — Ray Optics and Optical Instruments

Compound Microscope: Magnifying Power

9.11.2

Compound Microscope: Magnifying Power

A compound microscope uses two convex lenses in place of the simple microscope's single lens, to reach far higher magnifying powers than a single short-focal-length lens safely can. The lens nearer the (very small) object, called the objective, has a very short focal length fof_o and is positioned just beyond its own focus from the object, so that it forms a real, inverted, and already substantially magnified image of the object a little way inside the instrument's tube. This real intermediate image then acts as the object for the second lens, the eyepiece, which has a somewhat longer focal length fef_e and is positioned so that this intermediate image falls just inside the eyepiece's own focus, so that the eyepiece works exactly like a simple microscope, taking the already-magnified intermediate image and magnifying it a second time into a large final virtual image viewed by the eye. The overall magnifying power of the compound microscope is the product of the two individual magnifications -- the objective's own linear (lateral) magnification mom_o of the intermediate image, multiplied by the eyepiece's own angular magnifying power MeM_e acting on that intermediate image as its object: M=mo×Me.M=m_o\times M_e. When the final image is adjusted to form at the eye's near point DD (the normal way a compound microscope is used), and writing LL for the distance between the objective's own focus and the eyepiece's own focus (very nearly equal, for a well-designed instrument, to the tube length -- the distance between the two lenses), this combination works out to the commonly-quoted approximate formula M≈Lfo(1+Dfe).M\approx\frac{L}{f_o}\left(1+\frac{D}{f_e}\right). Because LL is typically many times larger than either fof_o or $f_ …

Figure 1Ray diagram for image formation in a compound microscope, final image at the near point

What this figure shows. A horizontal principal axis with two thin convex-lens symbols (vertical lines with outward arrowheads) drawn on it some distance apart: a smaller one on the left labelled 'objective (fo, short)' and a larger one on the right labelled 'eyepiece (fe, longer)'. A small vertical upward arrow (the tiny object) stands on the axis just outside the objective's focus, to its left. Two rays from the tip of this object pass through the objective and are shown converging to cross at a point between the two lenses, closer to the eyepiece side, where a taller inverted vertical arrow (the real, inverted intermediate image, labelled 'real image formed by objective') is drawn -- this crossing point is positioned just inside the eyepiece's own focus (on the objective side). From the tip of this intermediate-image arrow, two more rays are drawn diverging outward through the eyepiece, emerging on the far right as two rays that, when extended backward with dashed lines to the left, meet at a still taller, same-orientation (still inverted relative to the original object, but now upright relative to the intermediate image) arrow drawn far to the left of the whole diagram at the near point distance, labelled 'final vir …