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Exercises · Q5

Q.If a plant shows a symptom which could develop due to deficiency of more than one nutrient, how would you find out experimentally, the real deficient mineral element?

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When a symptom (such as chlorosis) can be produced by deficiency of more than one element, visual inspection alone cannot reliably tell which element is actually lacking. A controlled experimental approach is needed.

Steps of the experimental method:

  1. List the suspected elements that are each independently known to be capable of producing the observed symptom (for example, chlorosis can be caused by deficiency of N, Mg, Fe, Mn, Zn, K or S).
  2. Set up hydroponic (solution) cultures: prepare a series of nutrient solutions, each one complete in every element except a different one of the suspects, plus one fully complete "control" solution.
  3. Grow genetically identical, healthy plants of the same age and size in each solution under identical environmental conditions, so that the only variable between treatments is which element is missing.
  4. Observe and compare which treatment reproduces the exact symptom originally seen. The solution lacking the true culprit element will show that specific symptom, while the others (and the complete control) will show normal growth. …

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