Q.Which of the following stains is not used for staining chromosomes?
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🔒 Start your 14-day free trial to unlock the full solution →Concept understanding — Chromosome Morphology
Chromosome Morphology: What a Chromosome Actually Looks Like
Think of a chromosome as a tightly packed instruction manual. During most of a cell's life, that manual is loose and spread out — that's chromatin, the relaxed, stringy form of DNA wrapped around histone proteins. But when it's time for the cell to divide, the cell needs to move those instructions safely into two new cells. So it coils and condenses the chromatin into compact, rod-like structures we call chromosomes.
If you look at a condensed chromosome under a microscope, you see a distinct shape. It looks like two identical sticks joined at a pinched point. That pinched point is the centromere.
The Centromere: The Grip Point
The centromere is not just a visual feature — it's the functional anchor. During cell division, spindle fibres attach to a protein structure on the centromere called the kinetochore. The kinetochore is the actual "handle" that the spindle fibres grab to pull the chromosome apart. Without the centromere and kinetochore, the chromosome can't move, and division fails.
The centromere's position along the chromosome is fixed for that chromosome. And that position determines the chromosome's morphology — its shape and arm lengths.
The Four Types: Where the Centromere Sits
The centromere divides the chromosome into two arms: a short arm (called the p arm, from "petite") and a long arm (called the q arm, from "queue", French for tail). Depending on where the centromere lies, we get four categories:
| Type | Centromere Position | Arm Lengths | Shape |
|---|---|---|---|
| Metacentric | Exactly in the middle | p arm = q arm | V or X shape |
| Sub-metacentric | Slightly off-centre | p arm < q arm | L shape |
| Acrocentric | Very close to one end | p arm very short, q arm very long | J shape |
| Telocentric | At the very end | Only one arm visible | I shape |
In humans, telocentric chromosomes do not exist. The closest we have are acrocentric chromosomes (like chromosomes 13, 14, 15, 21, 22), which have such tiny p arms that they're often hard to see.
Visualising the Difference
Draw a straight line. Mark a point on it:
- Metacentric: point at the centre. The two halves are equal. When pulled apart during anaphase, the chromosome looks like an X.
- Sub-metacentric: point a bit to one side. One arm is noticeably longer. The chromosome looks like an L during movement.
- Acrocentric: point very near one end. One arm is a stub, the other is long. The chromosome looks like a J.
- Telocentric: point at the very end. Only one arm exists. The chromosome looks like a straight I.
Why This Matters …
Of the listed stains, methylene green is the one not used to stain chromosomes.
- Basic fuchsin, safranin and carmine (commonly used as acetocarmine) are classic dyes used in the laboratory to stain chromosomal material so it becomes visible under the microscope. …
Basic fuchsin, safranin and carmine are all standard chromosome stains; methylene green is not typically used for this purpose.
To see the fine detail of a chromosome under a light microscope, a stain that binds strongly to the condensed nucleoprotein material is needed. Basic fuchsin (used in the classic Feulgen staining reaction), safranin, and carmine (most often applied as acetocarmine) are the traditional dyes that laboratories use for exactly this purpose, because they bind well to chromosomal material and make its shape and banding pattern stand out clearly. …
Method: Recognise the Odd One Out by Association, Not by Memorised Definition
Questions that ask you to spot the one stain, reagent, or technique that doesn't belong in a list are usually testing recognition through repeated exposure rather than a precise technical definition — so the practical approach is to ask, for each option, "have I seen this name specifically paired with chromosome-staining in my notes?"
Basic fuchsin, safranin, and carmine (most often used in its acetic-acid form, acetocarmine) are all names that come up repeatedly and specifically in the context of preparing chromosome squashes for microscopy — they're the "usual suspects" for this particular lab technique. Methylene green doesn't carry that same specific association in this chapter's context, which is the clue that it's the odd one out here. …
- AP EAPCET 2024Set ap-2024-05-16-FN1 markMCQQ.Assertion (A): Satellite is a small fragment like structure found in some chromosomes Reason (R): It is a non staining secondary construction at a constant location (A) A and R are correct. R is the correct explanation of A (B) A and R are correct. R is not the correct explanation of A (C) A is correct but R is incorrect (D) A is incorrect but R is correct
›Reveal solutionSolution
A satellite is the small chromosomal segment lying distal to a secondary constriction; since the secondary constriction (non-staining, at a fixed location) is exactly what demarcates that segment, R correctly explains A.
Concept and Intuition
Certain chromosomes (SAT-chromosomes) have, in addition to the primary constriction (centromere), a second narrow, non-staining region called the secondary constriction, located at a constant position. The small chromosome segment lying beyond (distal to) this secondary constriction is called the satellite.
Step-by-Step Solution
- Assertion: a satellite is indeed a small, fragment-like structure seen attached to certain chromosomes (SAT-chromosomes) — a standard, correct cytogenetic fact.
- Reason: the secondary constriction is a non-staining, narrow region occurring at a constant (fixed) location on the chromosome — also a standard, correct fact; it is often associated with the nucleolar organizer region. …
- AP EAPCET 2023Set ap-2023-05-22-AN1 markMCQQ.In the chromatin the charge of a DNA and associated proteins, number of types of histones and copies are A. Positive, Negative B. Negative, Positive C. 4, 2 D. 16, 4 (A) B, D (B) B, C (C) A, C (D) A, D
›Reveal solutionSolution
DNA is negatively charged and histones are positively charged (statement B); the core histone octamer has 4 histone types, 2 copies each (statement C) — giving the answer B, C.
Concept and Intuition
Chromatin's basic packaging unit, the nucleosome, works because the negatively-charged DNA phosphate backbone is electrostatically attracted to and wound around a core of positively-charged histone proteins, neutralizing charge and enabling compact packaging.
Step-by-Step Solution
- DNA's sugar-phosphate backbone carries a net negative charge (phosphate groups); histones are rich in basic amino acids (lysine, arginine) giving them a net positive charge — matching item B ("Negative, Positive").
- The nucleosome core particle is an octamer built from two copies each of four distinct histone types — H2A, H2B, H3, and H4 — i.e., 4 types, 2 copies each, matching item C ("4, 2").
- H1 is a fifth "linker" histone, not part of the core octamer, and is not being counted here. …
- AP EAPCET 2023Set ap-2023-05-22-FN1 markMCQQ.The structures that appear as “Beads-on-string” in the chromatin and the number of base pairs are (A) Nucleotides, 100 (B) Genes, 200 (C) Kinetochore, 100 (D) Nucleosome, 200
›Reveal solutionSolution
The repeating "bead" units seen along chromatin fibres are nucleosomes, each wrapping roughly 200 base pairs of DNA around a histone octamer.
Concept and Intuition
DNA packaging in eukaryotic chromatin begins with winding the double helix around a core of eight histone proteins (two copies each of H2A, H2B, H3, H4), forming a nucleosome. Consecutive nucleosomes connected by short linker DNA segments give chromatin the classic "beads-on-a-string" look under the electron microscope. Each nucleosome core, together with its associated linker DNA, corresponds to about 200 base pairs.
Step-by-Step Solution
- Recall the packaging hierarchy of DNA: DNA → nucleosome → 30 nm chromatin fibre → looped domains → chromosome.
- Nucleosomes are the fundamental repeating unit responsible for the "beads-on-a-string" morphology. …
- AP EAPCET 2023Set ap-2023-05-22-FN1 markMCQQ.A non-staining secondary constriction at a constant location of few chromosomes is called (A) Centromere (B) Short arm (C) Satellite (D) Kinetochore
›Reveal solutionSolution
A constant, non-staining secondary constriction marks off a small terminal segment of the chromosome called the satellite.
Concept and Intuition
Besides the primary constriction (where the centromere is located), some chromosomes show a second, narrower non-staining region at a fixed position — this is the secondary constriction. It demarcates a small segment of the chromosome beyond it, known as the satellite, and such chromosomes are termed "SAT-chromosomes." This is different from the centromere (primary constriction) or the kinetochore (the protein structure at the centromere where spindle fibres attach).
Step-by-Step Solution
- Identify the described feature: non-staining, secondary constriction, fixed/constant position on specific chromosomes.
- This matches the definition of the segment/region called satellite, giving rise to "SAT-chromosomes." …
- AP EAPCET 2023Set ap-2023-05-22-FN1 markMCQQ.Assertion (A): Loss or gain of a segment of DNA results in alteration in chromosomes Reason (R) : Alterations in chromosomes do not result in abnormalities (A) Both A and R are correct and R is the correct explanation of A. (B) Both A and R are correct but R is not the correct explanation of A. (C) A is correct but R is incorrect. (D) A is incorrect but R is correct.
›Reveal solutionSolution
A correctly describes what a chromosomal aberration is; R wrongly denies that such aberrations cause abnormalities, when in fact they are a major cause of genetic disorders.
Concept and Intuition
Chromosomal mutations (aberrations) change either the structure or the number of chromosomes. Structural aberrations include deletion (loss of a segment), duplication (gain of a segment), inversion, and translocation. Because a chromosome segment carries many genes, losing or gaining it changes gene dosage or disrupts genes at the breakpoints — this is why such changes are clinically significant.
Step-by-Step Solution
- Evaluate A: loss/gain of a DNA segment = deletion/duplication, both textbook examples of chromosomal alteration. A is TRUE. …
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