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Exercises · 4.6

Q.Using a Punnett Square, workout the distribution of phenotypic features in the first filial generation after a cross between a homozygous female and a heterozygous male for a single locus.

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A cross between a homozygous female and a heterozygous male produces a 1:1 phenotypic ratio in the F₁ generation if the female is homozygous recessive, or all dominant phenotypes if she is homozygous dominant.

Understanding the Cross

Before we construct the Punnett square, we need to clarify what alleles each parent carries. A homozygous individual has two identical alleles at a locus—either both dominant (represented as AA) or both recessive (aa). A heterozygous individual carries one dominant and one recessive allele (Aa).

The question doesn't specify whether the homozygous female is dominant or recessive, so we'll work through both scenarios because they yield very different outcomes. This distinction matters enormously in Mendelian genetics.

Scenario 1: Homozygous Dominant Female (AA) × Heterozygous Male (Aa)

Let's set up the Punnett square. The female can only contribute A gametes, while the male produces both A and a gametes in equal proportions.

A (male)a (male)
A (female)AAAa
A (female)AAAa

The F₁ offspring are:

  • 50% AA (homozygous dominant)
  • 50% Aa (heterozygous)

Since both genotypes contain at least one dominant allele, all offspring display the dominant phenotype. The phenotypic ratio is therefore 100% dominant, or simply all uniform.

Scenario 2: Homozygous Recessive Female (aa) × Heterozygous Male (Aa)

Now the female contributes only a gametes, while the male still produces A and a in equal measure.

A (male)a (male)
a (female)Aaaa
a (female)Aaaa

The F₁ offspring are:

  • 50% Aa (heterozygous, showing dominant phenotype)
  • 50% aa (homozygous recessive, showing recessive phenotype)

Here we see a 1:1 phenotypic ratio—half the offspring express the dominant trait, half express the recessive trait.

Note

This 1:1 ratio is characteristic of a test cross, which Mendel used to determine whether an individual showing the dominant phenotype was homozygous or heterozygous. If any recessive offspring appear, the parent must have been heterozygous. …

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