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Question 87 of 96

Q.Describe the experiment carried out by Hershey and Chase. Write the conclusion they arrived at.

(OR)
(a) Describe the observations made on collection of white winged moths and dark winged moths in England between the years 1850 and 1920. What did these observations lead to ?
(b) How is the use of herbicides, pesticides and antibiotics by humans for various purposes, comparable with the observations made on moths in the above question ? What is this type of phenomenon called ?
Rajasthan RbseCBSE Class XII Board 2020Subjective· 5mImportance★★★★★
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Part (a): Hershey and Chase labelled phage DNA with 32P and protein with 35S; only DNA entered the bacterium, proving DNA is the genetic material.

Part (b): Peppered-moth colour change (industrial melanism) and resistance to herbicides/pesticides/antibiotics are both natural selection — a favourable variant increases under selective pressure.

Part (a)

Hershey and Chase (1952) used bacteriophages to settle whether DNA or protein carries heredity. DNA has phosphorus but no sulphur; protein has sulphur but no phosphorus.

  • Phages grown on 32P had radioactive DNA.
  • Phages grown on 35S had radioactive protein coats.

The labelled phages were allowed to attach to and infect E. coli. The mixture was agitated in a blender to detach the phage coats and then centrifuged. The heavier bacterial cells settled as a pellet.

Result: the 32P (DNA) was recovered with the bacterial pellet — it had entered the cells — while the 35S (protein) remained in the supernatant, outside the cells. …

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