Botany · Ch 13 — Photosynthesis
Photosynthetic Pigments
Photosynthetic Pigments
A photosynthetic pigment is any light-absorbing molecule housed in a chloroplast (or, in bacteria, in an equivalent photosynthetic structure) that captures the light energy photosynthesis runs on. These pigments fall into three chemically distinct families, summarised in Table 13.1: the chlorophylls (the primary, magnesium-centred pigments, covered in 13.3.1), the carotenoids (accessory, protective tetraterpenoid pigments, covered in 13.3.2), and the phycobilins (water-soluble accessory pigments found in cyanobacteria and red algae, covered in 13.3.3). All of these pigments except chlorophyll a itself function as accessory pigments - they absorb light at wavelengths chlorophyll a absorbs only weakly, and then transfer that captured energy on to chlorophyll a at the reaction centre, effectively widening the range of light a plant can usefully capture. Because the different pigments differ in molecular size and in how strongly they bind to chromatography paper, they can be physically separated and visualised by paper chromatography, …
| Chlorophyll | Carotenoids | Phycobilins |
|---|---|---|
| 1. Chlorophyll 'a' (C55H72O5N4Mg) - Green plants and Cyanobacteria | 1. Carotene (C40H56) - Lycopene (Red) | 1. Phycocyanin - Cyanobacteria |
| 2. Chlorophyll 'b' (C55H70O6N4Mg) - Green algae and all higher plants | 2. Xanthophyll (C40H56O2) - Yellow colour - Violaxanthin, Fucoxanthin (Brown Algae) and Lutein | 2. Phycoerythrin - Red Algae |
| 3. Chlorophyll 'c' (C55H32O5N4Mg) - Dinoflagellates, Diatoms and Brown Algae | ||
| 4. Chlorophyll 'd' - Red Algae | ||
| 5. Chlorophyll 'e' - Xanthophycean Algae | ||
| 6. Bacteriochlorophyll 'a' |
What this figure shows. A step-by-step chromatography set-up: chlorophyll pigment is first extracted from leaves using 80% acetone and concentrated by evaporation; a concentrated drop is spotted about 2 cm from one end of a chromatography paper strip; a 9:1 petroleum ether-to-acetone solvent mixture is poured into a development chamber; the strip is stood in the chamber with its spotted end just touching the solvent surface. After about an hour the pigments have separated by how far the solvent carries each one up the paper, forming four distinct bands from top to bottom: carotenes (travel furthest), xanthophyll, chlorophyll a, and chlorophyll b (travels least), with the solvent front sho …