Q.An angular magnification (magnifying power) of 30X is desired using an objective of focal length 1.25 cm and an eyepiece of focal length 5 cm. How will you set up the compound microscope?
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Microscope Magnification
A compound microscope views very small, nearby objects using two lenses in sequence: the objective (near the object) and the eyepiece (near the eye). Its total magnifying power is the product of what each lens contributes.
How the two lenses work together
- The object sits just beyond the focus of the short-focal-length objective (fo), which forms a real, inverted, magnified image inside the tube.
- That real image falls just inside the focus of the eyepiece (fe), which acts as a simple magnifier, producing a large virtual, magnified final image for the eye.
Because each stage magnifies, the effects multiply:
M=mo×me
The objective's magnification
mo=uovo≈foL
where L is the tube length (roughly the distance between the objective's second focal point and the eyepiece's first focal point). The object sits close to fo, so this approximation holds for a well-designed microscope.
The eyepiece's magnification
The eyepiece behaves as a simple magnifier:
- Final image at the near point (D=25 cm, largest magnification):
me=1+feD
- Final image at infinity (relaxed eye, "normal adjustment"):
me=feD
Total magnifying power
Image at the near point: M=foL(1+feD)
Image at infinity: M=foL⋅feD
High magnification needs short fo and fe (both sit in denominators) and a large tube length L — this is why a microscope objective is always a very short-focus lens.
Worked example
Objective fo=1.0 cm, eyepiece fe=2.5 cm, tube length L=20 cm, near point D=25 cm. Find M with the final image at the near point.
mo=1.020=20,me=1+2.525=11
M=20×11=220 …
Why this formula?
Microscope Magnification: Why the Formula Holds
Let's build the understanding from first principles — not just memorizing formulas, but seeing why they work.
1. What Does "Magnification" Mean in a Microscope?
A microscope creates a larger apparent image of a tiny object. The total magnification is the product of two stages:
- Objective lens — creates a real, enlarged, inverted image of the specimen.
- Eyepiece (ocular) — acts as a simple magnifier to view that real image.
So:
Total magnification = (magnification by objective) × (magnification by eyepiece)
2. The Key Formula
For a compound microscope in normal adjustment (final image at infinity, relaxed eye):
M=Mo×Me=(foL)×(feD)
Where:
- fo = focal length of objective
- fe = focal length of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- D = near point distance of the eye (usually 25 cm)
3. Derivation of Objective Magnification Mo
Step 1: How the objective works
The objective lens forms a real, inverted, enlarged image of the specimen. The specimen is placed just outside its focal point (fo).
Step 2: Using the lens formula
For a thin lens:
vo1−uo1=fo1
(Using sign convention: uo is negative, vo is positive)
Step 3: The tube length approximation
In a standard microscope, the specimen is placed very close to fo, so:
- uo≈−fo (object just beyond focal point)
- The image is formed at the first focal point of the eyepiece, which is at a distance L from the second focal point of the objective.
Thus:
vo≈fo+L
Step 4: Magnification formula
Lateral magnification by objective:
Mo=∣uo∣vo≈fofo+L=1+foL
Since L≫fo in practice, 1 is negligible:
Mo≈foL
Why this makes sense: A shorter fo means the objective is more "powerful" — it bends light more sharply, creating a larger image at the fixed tube length.
4. Derivation of Eyepiece Magnification Me
Step 1: The eyepiece as a simple magnifier
The eyepiece takes the real image from the objective and acts like a magnifying glass. For relaxed eye (final image at infinity), the real image must be placed at the focal point of the eyepiece.
Step 2: Angular magnification
Angular magnification is defined as:
Me=angle subtended by object at near pointangle subtended by image at eye
For a simple magnifier with image at infinity:
Me=feD
Where D=25 cm (standard near point). …
Setup: form the final image at the near point D=25 cm (the arrangement that gives maximum magnifying power).
Eyepiece magnification.
me=1+feD=1+525=6
Required objective magnification.
mo=meM=630=5
Object placement at the objective (∣vo∣=5∣uo∣, fo=1.25 cm):
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251⇒∣uo∣=1.5 cm
so vo=5×1.5=7.5 cm.
Eyepiece object distance (ve=−25 cm, fe=5 cm): …
Form the final image at the near point (25 cm): the eyepiece gives me=6, so the objective must give mo=5. This places the object 1.5 cm from the objective and separates the two lenses by about 11.67 cm.
Given
fo=1.25 cm, fe=5 cm, desired magnifying power M=30, near point D=25 cm.
Step 1 — Eyepiece magnification (image at near point)
me=1+feD=1+525=6
Step 2 — Objective magnification
Since M=mo×me,
mo=meM=630=5
Step 3 — Object position at the objective
The objective forms a real, inverted image, so ∣vo∣=5∣uo∣. Using vo1−uo1=fo1 with uo<0, vo>0:
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251
∣uo∣=56×1.25=1.5 cm,vo=5×1.5=7.5 cm
The object sits 1.5 cm from the objective, just beyond its focus fo=1.25 cm.
Step 4 — Eyepiece object distance …
Method: Two-Lens Ray Diagram Approach for Compound Microscope Setup
This method uses the magnification formula for a compound microscope to determine the required tube length and lens positions.
Step 1: Recall the magnification formula
For a compound microscope in normal adjustment (final image at infinity), the total angular magnification is:
M=mo×me=(−foL)×(feD)
Where:
- M = total angular magnification (magnifying power)
- mo = linear magnification of objective
- me = angular magnification of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- fo = focal length of objective = 1.25 cm
- fe = focal length of eyepiece = 5 cm
- D = least distance of distinct vision = 25 cm (standard value)
Step 2: Substitute known values
Given M=30X:
30=(−1.25L)×(525)
30=(−1.25L)×5
Step 3: Solve for tube length L
−1.25L=530=6
L=−6×1.25=−7.5 cm
The negative sign indicates the image formed by the objective is real and inverted (as expected). The magnitude gives:
Tube length L=7.5 cm
Step 4: Determine lens positions
The tube length L is the distance between: …
Here are the common mistakes students make when solving this compound microscope problem, along with how to avoid each.
Mistake 1: Confusing Magnification for the Final Image at Infinity vs. at Near Point
The error:
Students often blindly use the formula for angular magnification when the final image is at infinity (M=foL⋅feD) without checking the problem’s condition. Here, the desired magnification is 30X, but using the infinity formula gives a different tube length.
How to avoid:
Always check which case is implied. In most exam problems, unless stated otherwise, the final image is formed at the near point (25 cm). The correct formula for that case is:
M=foL(1+feD)
where D=25 cm (least distance of distinct vision).
For this problem, using fo=1.25 cm, fe=5 cm, and M=30, you solve for L:
30=1.25L(1+525)=1.25L×6
⇒L=630×1.25=6.25 cm
So the tube length is 6.25 cm.
Mistake 2: Forgetting to Add the Eyepiece Focal Length to Get Total Microscope Length
The error:
Students stop after finding L (distance between the second focal point of the objective and the first focal point of the eyepiece) and report that as the total length of the microscope.
How to avoid:
The total length of the microscope is the distance between the objective and the eyepiece. This is:
Total length=fo+L+fe
For this problem:
Total length=1.25+6.25+5=12.5 cm
Always draw a quick ray diagram to remind yourself: the objective’s second focal point and the eyepiece’s first focal point coincide — so the physical separation includes both focal lengths.
Mistake 3: Using the Wrong Sign Convention for Lens Formula
The error:
When verifying the image distances, students plug values into the lens formula without consistent sign convention, leading to negative distances that confuse them.
How to avoid:
Use the Cartesian sign convention (distances measured from the optical centre, positive in the direction of incident light). For the objective:
- uo is negative (object is real, on left)
- fo is positive (convex lens)
- vo is positive (real image on right)
For the eyepiece:
- ue is negative (object is real, on left)
- fe is positive
- ve is negative (final virtual image on left)
This consistency prevents sign errors.
--- …
- TG EAPCET 2026Set ap-2026-05-04-FN1 markMCQQ.A thin convex lens forms a virtual image when an object is placed on the principal axis at a distance of 12 cm from the lens. When the object is moved away from the lens along the principal axis through a distance of 18 cm, the lens forms a real image. If the magnification of the virtual image is five times the magnification of the real image, then the focal length of the lens is (A) 27 cm (B) 18 cm (C) 15 cm (D) 12 cm
›Reveal solutionSolution
Using m=f+uf for both object positions with ∣mvirtual∣=5∣mreal∣ gives f=15 cm. Option (C).
Set-up. A convex lens forms a virtual image when the object is inside the focus (∣u∣<f), and a real image when it is beyond the focus. With the Cartesian convention:
- Position 1 (virtual): u1=−12 cm
- Position 2 (real): u2=−(12+18)=−30 cm
Magnification. From v1−u1=f1 and m=uv:
m=f+uf.
For the virtual image (f>12, so this is positive):
m1=f−12f,∣m1∣=f−12f.
For the real image (f<30, so f−30<0, giving a negative value):
m2=f−30f,∣m2∣=30−ff.
Apply the condition ∣m1∣=5∣m2∣: …
- TG EAPCET 2026Set ap-2026-05-05-FN1 markMCQQ.Two thin convex lenses A and B of focal lengths 20 cm and 30 cm respectively are placed coaxially in air with a separation between them. If an object is placed in front of lens A at a distance of 45 cm from lens B, then the final image is formed at a distance of 30 cm from lens B. The distance between the two lenses is (A) 12 cm (B) 18 cm (C) 25 cm (D) 15 cm
›Reveal solutionSolution
With separation d=25 cm, the object sits exactly at the focus of lens A, so light leaves A parallel and lens B forms the image at its own focal length (30 cm) — matching the data.
Light passes object → lens A (fA=20 cm) → lens B (fB=30 cm). Let the lens separation be d. The object is 45 cm from lens B, and lens A lies d from lens B, so the object distance for A is
uA=45−d. …
- TG EAPCET 2025Set eng-2025-05-03-AN1 markMCQQ.A Cassegrain telescope uses two mirrors of radii of curvature 25 cm and 16 cm separated by a distance of 2.5 cm. The position of the final image of an object at infinity is (A) 40 cm from convex mirror (B) 4.44 cm from concave mirror (C) 4.44 cm from convex mirror (D) 40 cm from concave mirror
›Reveal solutionSolution
The final image forms 40 cm behind the convex secondary mirror — option (A).
Concept
In a Cassegrain telescope, parallel rays from an object at infinity first reflect off the large concave primary mirror and converge toward its focus. The small convex secondary mirror, placed short of that focus, intercepts the converging rays, so the primary's image acts as a virtual object for the secondary.
Solution
Focal lengths. Concave primary: R1=25 cm⇒f1=12.5 cm. Convex secondary: R2=16 cm⇒f2=8 cm.
Image from the primary. For an object at infinity the primary converges the rays to its focus, 12.5 cm in front of it.
Virtual object for the secondary. The mirrors are separated by d=2.5 cm, so the converging rays would meet at
12.5−2.5=10 cm
behind the secondary — a virtual object at 10 cm. …
- TG EAPCET 2025Set eng-2025-05-03-FN1 markMCQQ.A Cassegrain telescope uses two mirrors of radii of curvature 25 cm and 16 cm separated by a distance of 2.5 cm. The position of the final image of an object at infinity is (A) 40 cm from convex mirror (B) 4.44 cm from convex mirror (C) 4.44 cm from concave mirror (D) 40 cm from concave mirror
›Reveal solutionSolution
The concave primary aims light at its focus (12.5 cm); the convex secondary, 2.5 cm away, treats that as a virtual object 10 cm behind it and forms the final image 40 cm from itself. The correct option is (A) 40 cm from convex mirror.
Concept
In a Cassegrain telescope the concave primary mirror converges the parallel rays from a distant object toward its focus. Before the rays meet, the small convex secondary mirror intercepts them, so the converging beam acts as a virtual object for the secondary.
Primary (concave) mirror
f1=2R1=225=12.5 cm
For an object at infinity, the image would form 12.5 cm from the primary.
Secondary (convex) mirror
The mirrors are 2.5 cm apart, so this converging point lies
12.5−2.5=10 cm
behind the secondary — a virtual object at u=+10 cm. The convex mirror has …
- TG EAPCET 2025Set ap-2025-04-29-FN1 markMCQQ.A convex lens produces clear images when placed at two positions between an object and a screen that are 1 m apart. If the distance between the two positions of the lens at which clear images are formed is 20 cm, the focal length of the lens is (A) 5 cm (B) 24 cm (C) 125 cm (D) 12 cm
›Reveal solutionSolution
This is the displacement method for finding focal length. The lens forms two sharp images when moved between object and screen; the focal length is f=4DD2−d2, where D=100 cm and d=20 cm, giving f=24 cm.
The problem describes a classic experimental setup: an object and a screen are fixed at a separation D, and a convex lens is moved between them. At two distinct positions of the lens, a sharp image is formed on the screen. This is the displacement method (also called the conjugate foci method). The key insight is that for a fixed object-screen distance D>4f, there are two lens positions that satisfy the lens formula — one giving a magnified image, the other a diminished image. The distance between these two positions is d.
Why does this happen? For a given D, if the object distance is u, the image distance is D−u. The lens formula f1=u1+D−u1 becomes a quadratic in u, yielding two real solutions when D>4f. The difference between these two u values is exactly d.
The standard result is:
f=4DD2−d2
Let’s apply it step by step.
-
Identify the given distances.
The object and screen are 1 m apart, so D=100 cm.
The distance between the two lens positions is d=20 cm.
-
Plug into the formula.
f=4DD2−d2=4×1001002−202
- Simplify the numerator. …
-
- TG EAPCET 2024Set eng-2024-05-09-FN1 markMCQQ.For a combination of two convex lenses of focal lengths ‘f1’ and ‘f2’ to act as a glass slab, the distance of separation between them is (A) f1+f2 (B) f1−f2 (C) \dfrac{f_1 + f_2}{2} (D) \dfrac{f_1 - f_2}{2}
›Reveal solutionSolution
For two convex lenses to behave as a glass slab (i.e., zero net power and no deviation of a parallel beam), the separation must equal the sum of their focal lengths: d=f1+f2. The correct option is (A).
The key idea is that a glass slab does not converge or diverge light — it simply displaces the beam sideways without changing its overall direction. For a combination of two lenses to mimic this, the system must have zero net power and also produce no net deviation for a parallel incident beam. That happens when the second lens exactly cancels the convergence of the first, which requires the lenses to be placed so that the image formed by the first lens lies at the focal point of the second.
Let’s work through the reasoning step by step.
- Understand what “act as a glass slab” means. A glass slab (a plane-parallel plate) does not change the direction of a parallel beam; it only shifts it laterally. In lens terms, this means the combination must have zero effective focal length (infinite focal length) — i.e., the system’s power P=1/F=0. For two thin lenses separated by distance d, the effective focal length F is given by:
F1=f11+f21−f1f2d
Setting 1/F=0 gives:
f11+f21=f1f2d
Multiply through by f1f2:
f2+f1=d
So d=f1+f2. This is the condition for zero net power.
-
Check the physical meaning: ray tracing.
Imagine a parallel beam entering the first lens. It converges toward its focal point F1 (at distance f1 behind lens 1). If the second lens is placed exactly so that this converging point lies at its own focal point F2 (on the object side), then rays entering the second lens appear to come from its focal point, and thus emerge parallel again. The condition for this is that the distance between the lenses equals f1+f2 — the first lens’s focal length plus the second lens’s focal length. The beam is then undeviated overall, just shifted sideways — exactly like a glass slab.
-
Why the other options fail. …
- TG EAPCET 2022Set eng-2022-07-18-AN1 markMCQQ.A convex lens focusses an object 20 cm from it on a screen placed 5 cm away from it. A glass plate (refractive index =57) of thickness 1.4 cm is inserted between the lens and the screen. What is the distance of the object from the lens, so that its image is again focused on the screen? (A) 22.5 cm (B) 30.7 cm (C) 25.0 cm (D) 28.4 cm
›Reveal solutionSolution
The glass plate shifts the image by an apparent distance due to refraction; the lens must now form the image at a different virtual position to compensate. The required object distance is 25.0 cm, option (C).
The core idea here is that inserting a glass plate between the lens and the screen changes the effective optical path. The screen is fixed, but the plate makes the image appear to be at a different location from the lens’s perspective. You need to find where the lens should form the image so that, after the plate shifts it, the real image lands exactly on the screen.
Why does a plate shift the image? When light passes through a parallel-sided slab, the rays emerge parallel to their original direction but displaced sideways. For a thin slab placed close to the screen, the effect is that the image appears to move toward the lens by an amount equal to the slab’s thickness times (1−1/μ). This is a standard result — the apparent shift in the direction of the incident light is t(1−μ1).
Let’s work it through.
- First situation (no plate): Object distance u=−20 cm (sign convention: distances measured from lens, object on left is negative). Image distance v=+5 cm (real image on right). Using the lens formula:
f1=v1−u1=51−−201=51+201=204+1=205
So f=4 cm. That’s the focal length of the lens.
- Second situation (plate inserted): The plate of thickness t=1.4 cm and refractive index μ=7/5 is placed between the lens and the screen. The screen is still 5 cm from the lens. The plate shifts the image away from the screen (i.e., toward the lens) by an apparent distance. For a real image formed by the lens, the rays converge to a point; after passing through the plate, the convergence point shifts by:
Shift=t(1−μ1)=1.4(1−75)=1.4×72=0.4 cm
This shift is toward the lens — meaning the lens must now form the image 0.4 cm farther from the screen (i.e., at v′=5+0.4=5.4 cm from the lens) so that after the plate, the real image lands exactly on the screen.
Watch outA common mistake is to subtract the shift from the screen distance. But the plate makes the image appear closer to the lens, so the lens must send the image farther to compensate. Always check the direction: the shift is in the direction of light travel, so the image moves toward the lens.
- Find the new object distance: Using the lens formula again with f=4 cm and v′=+5.4 cm:
u′1=v′1−f1=5.41−41
Compute:
5.41=5410=275,41=41
u′1=275−41=10820−27=−1087
So u′=−7108≈−15.43 cm. That’s the object distance from the lens — but wait, that’s not among the options. Something’s off.
TipThe plate is between the lens and the screen, so the image formed by the lens is real and lies beyond the plate. The shift formula t(1−1/μ) applies when the object (here, the image from the lens) is in a medium of refractive index 1 (air) and the plate is the last medium before the screen. But careful: the shift is the apparent movement of the image as seen from the screen side. The lens must form the image at a distance v′ such that after the plate, the final image is at the screen. The correct relation is:
v′=v+t(1−μ1)
where v=5 cm is the screen distance. So v′=5+0.4=5.4 cm, as above. That gives u′≈−15.4 cm, which is not in the options. So perhaps the plate is placed between the object and the lens? Let’s re-read the problem.
The problem says: “A glass plate … is inserted between the lens and the screen.” That’s clear. But the answer options are around 25–30 cm, much larger than 15.4 cm. So maybe the plate shifts the image away from the lens? Let’s check the shift direction again. …
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