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Q.Mendel crossed a homozygous pea plant having yellow and round seeds with another pea plant bearing green and wrinkled seeds. He found that in some of the F2 population new combination of parental characters were observed. How will you explain the appearance of a new combination of parental characters in F2-offsprings ? Support your answer with the help of Punnett square.

(OR)
Describe S.L. Miller's experiment. Comment on the observations he made and his contribution towards the origin of life on Earth.
CBSECBSE Class XII Board 2019Subjective· 5mImportance★★★★★
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Part (a): the new combinations (yellow-wrinkled, green-round) arise by Mendel's law of independent assortment, which makes the YyRr F1 form four equally frequent gametes and yields a 9:3:3:1 F2 ratio. Part (b): Miller sparked CH₄, NH₃, H₂ and water vapour to mimic the early reducing atmosphere and lightning, obtained amino acids, and gave experimental support to the Oparin–Haldane theory of chemical evolution.

Part (a)

Mendel here followed two characters together — seed colour (yellow/green) and seed shape (round/wrinkled) — a dihybrid cross.

  • Parents: homozygous yellow-round (YYRR) × homozygous green-wrinkled (yyrr).
  • F1: all YyRr, expressing the dominant phenotypes yellow and round.

The decisive step is gamete formation in the F1. Because the colour gene and the shape gene lie on different chromosomes, they assort independently: how Y/y separates has no bearing on how R/r separates. So each F1 plant produces four gamete types in equal proportion — YR, Yr, yR, yr. If the two genes had stayed linked together, only the parental gametes YR and yr would form and no new combinations could appear.

Random fusion of these gametes is shown in the Punnett square:

YRYryRyr
YRYYRRYYRrYyRRYyRr
YrYYRrYYrrYyRrYyrr
yRYyRRYyRryyRRyyRr
yrYyRrYyrryyRryyrr

Counting phenotypes gives the classic 9:3:3:1 ratio:

  • 9 yellow-round (parental type)
  • 3 yellow-wrinkled (new combination)
  • 3 green-round (new combination)
  • 1 green-wrinkled (parental type) …

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