Q.A compound microscope consists of an objective lens of focal length 2.0 cm and an eyepiece of focal length 6.25 cm separated by a distance of 15 cm. How far from the objective should an object be placed in order to obtain the final image at
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Microscope Magnification
A compound microscope views very small, nearby objects using two lenses in sequence: the objective (near the object) and the eyepiece (near the eye). Its total magnifying power is the product of what each lens contributes.
How the two lenses work together
- The object sits just beyond the focus of the short-focal-length objective (fo), which forms a real, inverted, magnified image inside the tube.
- That real image falls just inside the focus of the eyepiece (fe), which acts as a simple magnifier, producing a large virtual, magnified final image for the eye.
Because each stage magnifies, the effects multiply:
M=mo×me
The objective's magnification
mo=uovo≈foL
where L is the tube length (roughly the distance between the objective's second focal point and the eyepiece's first focal point). The object sits close to fo, so this approximation holds for a well-designed microscope.
The eyepiece's magnification
The eyepiece behaves as a simple magnifier:
- Final image at the near point (D=25 cm, largest magnification):
me=1+feD
- Final image at infinity (relaxed eye, "normal adjustment"):
me=feD
Total magnifying power
Image at the near point: M=foL(1+feD)
Image at infinity: M=foL⋅feD
High magnification needs short fo and fe (both sit in denominators) and a large tube length L — this is why a microscope objective is always a very short-focus lens.
Worked example
Objective fo=1.0 cm, eyepiece fe=2.5 cm, tube length L=20 cm, near point D=25 cm. Find M with the final image at the near point.
mo=1.020=20,me=1+2.525=11
M=20×11=220 …
Why this formula?
Microscope Magnification: Why the Formula Holds
Let's build the understanding from first principles — not just memorizing formulas, but seeing why they work.
1. What Does "Magnification" Mean in a Microscope?
A microscope creates a larger apparent image of a tiny object. The total magnification is the product of two stages:
- Objective lens — creates a real, enlarged, inverted image of the specimen.
- Eyepiece (ocular) — acts as a simple magnifier to view that real image.
So:
Total magnification = (magnification by objective) × (magnification by eyepiece)
2. The Key Formula
For a compound microscope in normal adjustment (final image at infinity, relaxed eye):
M=Mo×Me=(foL)×(feD)
Where:
- fo = focal length of objective
- fe = focal length of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- D = near point distance of the eye (usually 25 cm)
3. Derivation of Objective Magnification Mo
Step 1: How the objective works
The objective lens forms a real, inverted, enlarged image of the specimen. The specimen is placed just outside its focal point (fo).
Step 2: Using the lens formula
For a thin lens:
vo1−uo1=fo1
(Using sign convention: uo is negative, vo is positive)
Step 3: The tube length approximation
In a standard microscope, the specimen is placed very close to fo, so:
- uo≈−fo (object just beyond focal point)
- The image is formed at the first focal point of the eyepiece, which is at a distance L from the second focal point of the objective.
Thus:
vo≈fo+L
Step 4: Magnification formula
Lateral magnification by objective:
Mo=∣uo∣vo≈fofo+L=1+foL
Since L≫fo in practice, 1 is negligible:
Mo≈foL
Why this makes sense: A shorter fo means the objective is more "powerful" — it bends light more sharply, creating a larger image at the fixed tube length.
4. Derivation of Eyepiece Magnification Me
Step 1: The eyepiece as a simple magnifier
The eyepiece takes the real image from the objective and acts like a magnifying glass. For relaxed eye (final image at infinity), the real image must be placed at the focal point of the eyepiece.
Step 2: Angular magnification
Angular magnification is defined as:
Me=angle subtended by object at near pointangle subtended by image at eye
For a simple magnifier with image at infinity:
Me=feD
Where D=25 cm (standard near point). …
Concept: Microscope Magnification — The final image is formed by the eyepiece, so we first find the intermediate image distance for the eyepiece, then use that to find the object distance for the objective.
Step 1: Eyepiece for case (a) — final image at D=25 cm
For the eyepiece, fe=6.25 cm, ve=−25 cm (virtual image). Using lens formula:
ue1=ve1−fe1=−251−6.251=−0.04−0.16=−0.20
So ue=−5 cm. The intermediate image is 5 cm from the eyepiece on the objective side.
Step 2: Objective for case (a)
Tube length L=15 cm, so vo=L−∣ue∣=15−5=10 cm.
Objective fo=2.0 cm. Using lens formula:
uo1=vo1−fo1=101−21=0.1−0.5=−0.4
Thus uo=−2.5 cm. Object is 2.5 cm from objective.
Step 3: Magnifying power for case (a)
M=−uovo(1+feD)=−−2.510(1+6.2525)=4×(1+4)=20
Step 4: Case (b) — final image at infinity …
Treating the objective and eyepiece as two lenses in sequence and working back from the required final-image position: (a) for the final image at the near point (25 cm) the object must be 2.5 cm from the objective, giving magnifying power 20;
(b) for the final image at infinity the object must be 2770≈2.59 cm from the objective, giving magnifying power 13.5.
How a compound microscope works
The objective (fo=2.0 cm) forms a real, enlarged, inverted intermediate image; the eyepiece (fe=6.25 cm) then acts as a simple magnifier on that image. The lenses are fixed L=15 cm apart. We work backward from the eyepiece, since the required position of the final image fixes where the intermediate image must sit.
Case (a): final image at the least distance of distinct vision, ve=−25 cm
Eyepiece. Using ve1−ue1=fe1 with ve=−25 cm, fe=6.25 cm:
ue1=ve1−fe1=−251−6.251=−0.04−0.16=−0.20⇒ue=−5.0 cm.
So the intermediate image is 5.0 cm in front of the eyepiece, i.e. 15−5.0=10.0 cm from the objective, giving vo=+10.0 cm.
Objective. Using vo1−uo1=fo1 with vo=10.0 cm, fo=2.0 cm:
uo1=vo1−fo1=101−21=−0.4⇒uo=−2.5 cm.
The object is placed 2.5 cm from the objective (just beyond its focus fo=2.0 cm, as expected).
Magnifying power. …
Method: Two-Step Image Formation (Ray Tracing by Lens Equations)
This problem treats the compound microscope as two lenses in series — the objective forms a real, inverted, enlarged image, and the eyepiece then magnifies that image further. We apply the thin lens formula to each lens in turn, using the fixed separation between the lenses.
Step 1 – Understand the layout
- Objective: fo=2.0 cm
- Eyepiece: fe=6.25 cm
- Separation between lenses: L=15 cm
- Final image distance from eyepiece:
- Case (a): ve=−25 cm (least distance of distinct vision, virtual image)
- Case (b): ve=∞ (image at infinity)
We use the Cartesian sign convention with the lens formula v1−u1=f1. The image formed by the objective acts as the object for the eyepiece; the two are linked by
∣vo∣+∣ue∣=L
Step 2 – Eyepiece, Case (a): final image at 25 cm
For the eyepiece, ve=−25 cm and fe=+6.25 cm:
ue1=ve1−fe1=−251−6.251=−0.04−0.16=−0.20
ue=−5 cm
So the objective’s image lies 5 cm in front of the eyepiece — a real object placed just inside the eyepiece’s focal length, which is exactly what produces a magnified virtual image at 25 cm.
The image distance for the objective is therefore
vo=L−∣ue∣=15−5=10 cm
Step 3 – Objective, Case (a)
Lens formula for the objective (fo=2.0 cm, vo=+10 cm):
uo1=vo1−fo1=101−21=0.1−0.5=−0.4
uo=−2.5 cm
The object must be placed 2.5 cm in front of the objective.
Step 4 – Magnifying power, Case (a)
With the final image at the least distance of distinct vision D=25 cm:
M=∣uo∣vo(1+feD)
∣uo∣vo=2.510=4,1+feD=1+6.2525=1+4=5
M=4×5=20
Step 5 – Case (b): final image at infinity …
Common Mistakes & How to Avoid Them
Mistake 1: Confusing the separation distance with image distances
The error: Students often take the given 15 cm separation as vo or ue directly, without realising it is the distance between the two lenses (L=vo+ue).
How to avoid:
- Draw a clear ray diagram.
- Label the objective-to-eyepiece distance as L=vo+ue.
- Never substitute 15 cm for vo alone — it is the sum of two distances.
Mistake 2: Forgetting sign conventions for the eyepiece
The error: Treating the eyepiece as a converging lens but forgetting that the final image is virtual (on the same side as the object for the eyepiece). This leads to wrong signs in the lens formula.
How to avoid:
- For the eyepiece, the final image distance ve is negative (virtual image).
- Case (a): ve=−D=−25 cm
- Case (b): ve=−∞
- Use the lens formula with correct signs:
fe1=ve1−ue1
(where ue is negative because the object for eyepiece is real and on the opposite side).
Mistake 3: Using the wrong formula for magnifying power
The error: Mixing up the two cases — using the near-point formula when the final image is at infinity, or vice versa.
How to avoid:
- Final image at near point (D=25 cm):
M=Mo×Me=(−uovo)×(1+feD)
- Final image at infinity:
M=Mo×Me=(−uovo)×(feD)
- Memorise: the +1 appears only when the eye is accommodating (near point).
Mistake 4: Forgetting the negative sign in magnification
The error: Reporting magnifying power as a positive number without indicating that the image is inverted relative to the object.
How to avoid:
- The objective magnification Mo=−vo/uo is negative (real, inverted image).
- The eyepiece magnification Me is positive (virtual, erect relative to the intermediate image).
- The total magnification M=Mo×Me is negative, meaning the final image is inverted.
- In exam answers, state: “Magnifying power = ∣M∣ (magnitude)” or explicitly say “image is inverted.”
Mistake 5: Not checking if the intermediate image lies within the eyepiece focal length …
- GSEB Higher Secondary Certificate (HSC) Examination 2026Set ANNUAL4 marksQ.A person with a normal near point (25 cm) using a compound microscope with objective of focal length 8.0 mm and an eyepiece of focal length 2.5 cm can bring an object placed at 9.0 mm from the objective in sharp focus. What is the separation between the two lenses? Calculate the magnifying power of the microscope.
›Reveal solutionSolution
First find where the objective lens forms its (real, magnified) intermediate image using the lens formula; then require the eyepiece to form the final image at the near point (25 cm) for maximum magnification, which fixes the eyepiece's object distance; the lens separation is the sum of the two image/object distances, and total magnification is the product of the two individual magnifications.
Given: near point D = 25 cm, objective focal length f_o = 8.0 mm = 0.8 cm, object distance from objective u_o = 9.0 mm = 0.9 cm (object just outside focal length, as required for a microscope objective), eyepiece focal length f_e = 2.5 cm.
Step 1 - Image formed by objective: Using the thin lens formula (sign convention: distances measured from the lens, object distance taken negative):
u_o = -0.9 cm, f_o = +0.8 cm (convex lens)
1/v_o - 1/u_o = 1/f_o
1/v_o = 1/f_o + 1/u_o = 1/0.8 + 1/(-0.9) = 1.25 - 1.111 = 0.139
v_o = 1/0.139 = 7.2 cm
So the objective forms a real, inverted, magnified image at 7.2 cm on the far side of the objective.
Step 2 - Eyepiece positioned for image at near point (maximum magnification, image at D): This intermediate image (from the objective) acts as the object for the eyepiece. For maximum magnifying power, the eyepiece should form its final (virtual) image at the near point, i.e. v_e = -25 cm (virtual image, same side as the object, by sign convention).
1/v_e - 1/u_e = 1/f_e
1/(-25) - 1/u_e = 1/2.5
-0.04 - 1/u_e = 0.4
-1/u_e = 0.44
u_e = -1/0.44 = -2.27 cm
So the intermediate image must be 2.27 cm in front of the eyepiece for the eyepiece to form the final image at the near point.
…
- GSEB Higher Secondary Certificate (HSC) Examination 2024Set ANNUAL4 marksQ.Draw the ray diagram for the formation of image by a compound microscope and obtained the formula for magnification.
›Reveal solutionSolution
Figure — The stem instructs 'Draw the ray diagram for the formation of image by a compound microscope'; the catalog fig A compound microscope's objective forms a real, magnified image which then acts as the object for the eyepiece, which forms a further-magnified virtual image; total magnification is the product of the two individual magnifications.
Ray diagram (described): The object AB is placed just beyond the focus Fo of the objective lens (between fo and 2fo). The objective forms a real, inverted, magnified image A'B' beyond 2Fo on the other side. This intermediate image A'B' falls within the focal length of the eyepiece (between the eyepiece and its focus Fe). The eyepiece then acts as a simple magnifier on this intermediate image, producing a final virtual, further-magnified, and (relative to the object) inverted image A''B'' — formed at the near point D for maximum magnification, or at infinity for normal (relaxed-eye) adjustment. Both lenses are convex, mounted coaxially at opposite ends of a tube, with the objective having a small aperture and focal length, and the eyepiece having a larger aperture.
…
- GSEB Higher Secondary Certificate (HSC) Examination 2023Set ANNUAL4 marksQ.A compound microscope consists of an objective lens of focal length 2.0 cm and an eye piece of focal length 6.25 cm separated by a distance 15 cm. How far from the objective should an object be placed in order to obtain the final image ata) the least distance of distinct vision (25 cm) andb) at infinity? What is the magnifying power of microscope in each case?
›Reveal solutionSolution
Work back from the eyepiece: (a) for image at 25 cm, u_e = -5 cm, objective forms image at 10 cm, u_o = -2.5 cm, M = 20; (b) for image at infinity, u_e = -6.25 cm, objective image at 8.75 cm, u_o = -2.59 cm, M = 13.5.
Data: f_o = 2.0 cm, f_e = 6.25 cm, tube separation L = 15 cm, D = 25 cm.
- Final image at least distance of distinct vision (25 cm): Eyepiece: image virtual at v_e = -25 cm. 1/v_e - 1/u_e = 1/f_e: 1/(-25) - 1/u_e = 1/6.25 => -1/u_e = 1/6.25 + 1/25 = 5/25 = 1/5 => u_e = -5 cm. So the objective's image is 5 cm in front of the eyepiece; distance from objective = L - 5 = 10 cm => v_o = +10 cm. Objective: 1/v_o - 1/u_o = 1/f_o: 1/10 - 1/u_o = 1/2 => -1/u_o = 1/2 - 1/10 = 2/5 => u_o = -2.5 cm. Magnifying power: M = m_o (1 + D/f_e) = (v_o/|u_o|)(1 + D/f_e) = (10/2.5)(1 + 25/6.25) = 4 x 5 = 20.
- Final image at infinity: …
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