Q.An angular magnification (magnifying power) of 30X is desired using an objective of focal length 1.25 cm and an eyepiece of focal length 5 cm. How will you set up the compound microscope?
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Microscope Magnification
A compound microscope views very small, nearby objects using two lenses in sequence: the objective (near the object) and the eyepiece (near the eye). Its total magnifying power is the product of what each lens contributes.
How the two lenses work together
- The object sits just beyond the focus of the short-focal-length objective (fo), which forms a real, inverted, magnified image inside the tube.
- That real image falls just inside the focus of the eyepiece (fe), which acts as a simple magnifier, producing a large virtual, magnified final image for the eye.
Because each stage magnifies, the effects multiply:
M=mo×me
The objective's magnification
mo=uovo≈foL
where L is the tube length (roughly the distance between the objective's second focal point and the eyepiece's first focal point). The object sits close to fo, so this approximation holds for a well-designed microscope.
The eyepiece's magnification
The eyepiece behaves as a simple magnifier:
- Final image at the near point (D=25 cm, largest magnification):
me=1+feD
- Final image at infinity (relaxed eye, "normal adjustment"):
me=feD
Total magnifying power
Image at the near point: M=foL(1+feD)
Image at infinity: M=foL⋅feD
High magnification needs short fo and fe (both sit in denominators) and a large tube length L — this is why a microscope objective is always a very short-focus lens.
Worked example
Objective fo=1.0 cm, eyepiece fe=2.5 cm, tube length L=20 cm, near point D=25 cm. Find M with the final image at the near point.
mo=1.020=20,me=1+2.525=11
M=20×11=220 …
Why this formula?
Microscope Magnification: Why the Formula Holds
Let's build the understanding from first principles — not just memorizing formulas, but seeing why they work.
1. What Does "Magnification" Mean in a Microscope?
A microscope creates a larger apparent image of a tiny object. The total magnification is the product of two stages:
- Objective lens — creates a real, enlarged, inverted image of the specimen.
- Eyepiece (ocular) — acts as a simple magnifier to view that real image.
So:
Total magnification = (magnification by objective) × (magnification by eyepiece)
2. The Key Formula
For a compound microscope in normal adjustment (final image at infinity, relaxed eye):
M=Mo×Me=(foL)×(feD)
Where:
- fo = focal length of objective
- fe = focal length of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- D = near point distance of the eye (usually 25 cm)
3. Derivation of Objective Magnification Mo
Step 1: How the objective works
The objective lens forms a real, inverted, enlarged image of the specimen. The specimen is placed just outside its focal point (fo).
Step 2: Using the lens formula
For a thin lens:
vo1−uo1=fo1
(Using sign convention: uo is negative, vo is positive)
Step 3: The tube length approximation
In a standard microscope, the specimen is placed very close to fo, so:
- uo≈−fo (object just beyond focal point)
- The image is formed at the first focal point of the eyepiece, which is at a distance L from the second focal point of the objective.
Thus:
vo≈fo+L
Step 4: Magnification formula
Lateral magnification by objective:
Mo=∣uo∣vo≈fofo+L=1+foL
Since L≫fo in practice, 1 is negligible:
Mo≈foL
Why this makes sense: A shorter fo means the objective is more "powerful" — it bends light more sharply, creating a larger image at the fixed tube length.
4. Derivation of Eyepiece Magnification Me
Step 1: The eyepiece as a simple magnifier
The eyepiece takes the real image from the objective and acts like a magnifying glass. For relaxed eye (final image at infinity), the real image must be placed at the focal point of the eyepiece.
Step 2: Angular magnification
Angular magnification is defined as:
Me=angle subtended by object at near pointangle subtended by image at eye
For a simple magnifier with image at infinity:
Me=feD
Where D=25 cm (standard near point). …
Setup: form the final image at the near point D=25 cm (the arrangement that gives maximum magnifying power).
Eyepiece magnification.
me=1+feD=1+525=6
Required objective magnification.
mo=meM=630=5
Object placement at the objective (∣vo∣=5∣uo∣, fo=1.25 cm):
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251⇒∣uo∣=1.5 cm
so vo=5×1.5=7.5 cm.
Eyepiece object distance (ve=−25 cm, fe=5 cm): …
Form the final image at the near point (25 cm): the eyepiece gives me=6, so the objective must give mo=5. This places the object 1.5 cm from the objective and separates the two lenses by about 11.67 cm.
Given
fo=1.25 cm, fe=5 cm, desired magnifying power M=30, near point D=25 cm.
Step 1 — Eyepiece magnification (image at near point)
me=1+feD=1+525=6
Step 2 — Objective magnification
Since M=mo×me,
mo=meM=630=5
Step 3 — Object position at the objective
The objective forms a real, inverted image, so ∣vo∣=5∣uo∣. Using vo1−uo1=fo1 with uo<0, vo>0:
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251
∣uo∣=56×1.25=1.5 cm,vo=5×1.5=7.5 cm
The object sits 1.5 cm from the objective, just beyond its focus fo=1.25 cm.
Step 4 — Eyepiece object distance …
Method: Two-Lens Ray Diagram Approach for Compound Microscope Setup
This method uses the magnification formula for a compound microscope to determine the required tube length and lens positions.
Step 1: Recall the magnification formula
For a compound microscope in normal adjustment (final image at infinity), the total angular magnification is:
M=mo×me=(−foL)×(feD)
Where:
- M = total angular magnification (magnifying power)
- mo = linear magnification of objective
- me = angular magnification of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- fo = focal length of objective = 1.25 cm
- fe = focal length of eyepiece = 5 cm
- D = least distance of distinct vision = 25 cm (standard value)
Step 2: Substitute known values
Given M=30X:
30=(−1.25L)×(525)
30=(−1.25L)×5
Step 3: Solve for tube length L
−1.25L=530=6
L=−6×1.25=−7.5 cm
The negative sign indicates the image formed by the objective is real and inverted (as expected). The magnitude gives:
Tube length L=7.5 cm
Step 4: Determine lens positions
The tube length L is the distance between: …
Here are the common mistakes students make when solving this compound microscope problem, along with how to avoid each.
Mistake 1: Confusing Magnification for the Final Image at Infinity vs. at Near Point
The error:
Students often blindly use the formula for angular magnification when the final image is at infinity (M=foL⋅feD) without checking the problem’s condition. Here, the desired magnification is 30X, but using the infinity formula gives a different tube length.
How to avoid:
Always check which case is implied. In most exam problems, unless stated otherwise, the final image is formed at the near point (25 cm). The correct formula for that case is:
M=foL(1+feD)
where D=25 cm (least distance of distinct vision).
For this problem, using fo=1.25 cm, fe=5 cm, and M=30, you solve for L:
30=1.25L(1+525)=1.25L×6
⇒L=630×1.25=6.25 cm
So the tube length is 6.25 cm.
Mistake 2: Forgetting to Add the Eyepiece Focal Length to Get Total Microscope Length
The error:
Students stop after finding L (distance between the second focal point of the objective and the first focal point of the eyepiece) and report that as the total length of the microscope.
How to avoid:
The total length of the microscope is the distance between the objective and the eyepiece. This is:
Total length=fo+L+fe
For this problem:
Total length=1.25+6.25+5=12.5 cm
Always draw a quick ray diagram to remind yourself: the objective’s second focal point and the eyepiece’s first focal point coincide — so the physical separation includes both focal lengths.
Mistake 3: Using the Wrong Sign Convention for Lens Formula
The error:
When verifying the image distances, students plug values into the lens formula without consistent sign convention, leading to negative distances that confuse them.
How to avoid:
Use the Cartesian sign convention (distances measured from the optical centre, positive in the direction of incident light). For the objective:
- uo is negative (object is real, on left)
- fo is positive (convex lens)
- vo is positive (real image on right)
For the eyepiece:
- ue is negative (object is real, on left)
- fe is positive
- ve is negative (final virtual image on left)
This consistency prevents sign errors.
--- …
- GSEB Higher Secondary Certificate (HSC) Examination 2026Set ANNUAL4 marksQ.A person with a normal near point (25 cm) using a compound microscope with objective of focal length 8.0 mm and an eyepiece of focal length 2.5 cm can bring an object placed at 9.0 mm from the objective in sharp focus. What is the separation between the two lenses? Calculate the magnifying power of the microscope.
›Reveal solutionSolution
First find where the objective lens forms its (real, magnified) intermediate image using the lens formula; then require the eyepiece to form the final image at the near point (25 cm) for maximum magnification, which fixes the eyepiece's object distance; the lens separation is the sum of the two image/object distances, and total magnification is the product of the two individual magnifications.
Given: near point D = 25 cm, objective focal length f_o = 8.0 mm = 0.8 cm, object distance from objective u_o = 9.0 mm = 0.9 cm (object just outside focal length, as required for a microscope objective), eyepiece focal length f_e = 2.5 cm.
Step 1 - Image formed by objective: Using the thin lens formula (sign convention: distances measured from the lens, object distance taken negative):
u_o = -0.9 cm, f_o = +0.8 cm (convex lens)
1/v_o - 1/u_o = 1/f_o
1/v_o = 1/f_o + 1/u_o = 1/0.8 + 1/(-0.9) = 1.25 - 1.111 = 0.139
v_o = 1/0.139 = 7.2 cm
So the objective forms a real, inverted, magnified image at 7.2 cm on the far side of the objective.
Step 2 - Eyepiece positioned for image at near point (maximum magnification, image at D): This intermediate image (from the objective) acts as the object for the eyepiece. For maximum magnifying power, the eyepiece should form its final (virtual) image at the near point, i.e. v_e = -25 cm (virtual image, same side as the object, by sign convention).
1/v_e - 1/u_e = 1/f_e
1/(-25) - 1/u_e = 1/2.5
-0.04 - 1/u_e = 0.4
-1/u_e = 0.44
u_e = -1/0.44 = -2.27 cm
So the intermediate image must be 2.27 cm in front of the eyepiece for the eyepiece to form the final image at the near point.
…
- GSEB Higher Secondary Certificate (HSC) Examination 2024Set ANNUAL4 marksQ.Draw the ray diagram for the formation of image by a compound microscope and obtained the formula for magnification.
›Reveal solutionSolution
Figure — The stem instructs 'Draw the ray diagram for the formation of image by a compound microscope'; the catalog fig A compound microscope's objective forms a real, magnified image which then acts as the object for the eyepiece, which forms a further-magnified virtual image; total magnification is the product of the two individual magnifications.
Ray diagram (described): The object AB is placed just beyond the focus Fo of the objective lens (between fo and 2fo). The objective forms a real, inverted, magnified image A'B' beyond 2Fo on the other side. This intermediate image A'B' falls within the focal length of the eyepiece (between the eyepiece and its focus Fe). The eyepiece then acts as a simple magnifier on this intermediate image, producing a final virtual, further-magnified, and (relative to the object) inverted image A''B'' — formed at the near point D for maximum magnification, or at infinity for normal (relaxed-eye) adjustment. Both lenses are convex, mounted coaxially at opposite ends of a tube, with the objective having a small aperture and focal length, and the eyepiece having a larger aperture.
…
- GSEB Higher Secondary Certificate (HSC) Examination 2023Set ANNUAL4 marksQ.A compound microscope consists of an objective lens of focal length 2.0 cm and an eye piece of focal length 6.25 cm separated by a distance 15 cm. How far from the objective should an object be placed in order to obtain the final image ata) the least distance of distinct vision (25 cm) andb) at infinity? What is the magnifying power of microscope in each case?
›Reveal solutionSolution
Work back from the eyepiece: (a) for image at 25 cm, u_e = -5 cm, objective forms image at 10 cm, u_o = -2.5 cm, M = 20; (b) for image at infinity, u_e = -6.25 cm, objective image at 8.75 cm, u_o = -2.59 cm, M = 13.5.
Data: f_o = 2.0 cm, f_e = 6.25 cm, tube separation L = 15 cm, D = 25 cm.
- Final image at least distance of distinct vision (25 cm): Eyepiece: image virtual at v_e = -25 cm. 1/v_e - 1/u_e = 1/f_e: 1/(-25) - 1/u_e = 1/6.25 => -1/u_e = 1/6.25 + 1/25 = 5/25 = 1/5 => u_e = -5 cm. So the objective's image is 5 cm in front of the eyepiece; distance from objective = L - 5 = 10 cm => v_o = +10 cm. Objective: 1/v_o - 1/u_o = 1/f_o: 1/10 - 1/u_o = 1/2 => -1/u_o = 1/2 - 1/10 = 2/5 => u_o = -2.5 cm. Magnifying power: M = m_o (1 + D/f_e) = (v_o/|u_o|)(1 + D/f_e) = (10/2.5)(1 + 25/6.25) = 4 x 5 = 20.
- Final image at infinity: …
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