Q.An angular magnification (magnifying power) of 30X is desired using an objective of focal length 1.25 cm and an eyepiece of focal length 5 cm. How will you set up the compound microscope?
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Microscope Magnification
A compound microscope views very small, nearby objects using two lenses in sequence: the objective (near the object) and the eyepiece (near the eye). Its total magnifying power is the product of what each lens contributes.
How the two lenses work together
- The object sits just beyond the focus of the short-focal-length objective (fo), which forms a real, inverted, magnified image inside the tube.
- That real image falls just inside the focus of the eyepiece (fe), which acts as a simple magnifier, producing a large virtual, magnified final image for the eye.
Because each stage magnifies, the effects multiply:
M=mo×me
The objective's magnification
mo=uovo≈foL
where L is the tube length (roughly the distance between the objective's second focal point and the eyepiece's first focal point). The object sits close to fo, so this approximation holds for a well-designed microscope.
The eyepiece's magnification
The eyepiece behaves as a simple magnifier:
- Final image at the near point (D=25 cm, largest magnification):
me=1+feD
- Final image at infinity (relaxed eye, "normal adjustment"):
me=feD
Total magnifying power
Image at the near point: M=foL(1+feD)
Image at infinity: M=foL⋅feD
High magnification needs short fo and fe (both sit in denominators) and a large tube length L — this is why a microscope objective is always a very short-focus lens.
Worked example
Objective fo=1.0 cm, eyepiece fe=2.5 cm, tube length L=20 cm, near point D=25 cm. Find M with the final image at the near point.
mo=1.020=20,me=1+2.525=11
M=20×11=220 …
Why this formula?
Microscope Magnification: Why the Formula Holds
Let's build the understanding from first principles — not just memorizing formulas, but seeing why they work.
1. What Does "Magnification" Mean in a Microscope?
A microscope creates a larger apparent image of a tiny object. The total magnification is the product of two stages:
- Objective lens — creates a real, enlarged, inverted image of the specimen.
- Eyepiece (ocular) — acts as a simple magnifier to view that real image.
So:
Total magnification = (magnification by objective) × (magnification by eyepiece)
2. The Key Formula
For a compound microscope in normal adjustment (final image at infinity, relaxed eye):
M=Mo×Me=(foL)×(feD)
Where:
- fo = focal length of objective
- fe = focal length of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- D = near point distance of the eye (usually 25 cm)
3. Derivation of Objective Magnification Mo
Step 1: How the objective works
The objective lens forms a real, inverted, enlarged image of the specimen. The specimen is placed just outside its focal point (fo).
Step 2: Using the lens formula
For a thin lens:
vo1−uo1=fo1
(Using sign convention: uo is negative, vo is positive)
Step 3: The tube length approximation
In a standard microscope, the specimen is placed very close to fo, so:
- uo≈−fo (object just beyond focal point)
- The image is formed at the first focal point of the eyepiece, which is at a distance L from the second focal point of the objective.
Thus:
vo≈fo+L
Step 4: Magnification formula
Lateral magnification by objective:
Mo=∣uo∣vo≈fofo+L=1+foL
Since L≫fo in practice, 1 is negligible:
Mo≈foL
Why this makes sense: A shorter fo means the objective is more "powerful" — it bends light more sharply, creating a larger image at the fixed tube length.
4. Derivation of Eyepiece Magnification Me
Step 1: The eyepiece as a simple magnifier
The eyepiece takes the real image from the objective and acts like a magnifying glass. For relaxed eye (final image at infinity), the real image must be placed at the focal point of the eyepiece.
Step 2: Angular magnification
Angular magnification is defined as:
Me=angle subtended by object at near pointangle subtended by image at eye
For a simple magnifier with image at infinity:
Me=feD
Where D=25 cm (standard near point). …
Setup: form the final image at the near point D=25 cm (the arrangement that gives maximum magnifying power).
Eyepiece magnification.
me=1+feD=1+525=6
Required objective magnification.
mo=meM=630=5
Object placement at the objective (∣vo∣=5∣uo∣, fo=1.25 cm):
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251⇒∣uo∣=1.5 cm
so vo=5×1.5=7.5 cm.
Eyepiece object distance (ve=−25 cm, fe=5 cm): …
Form the final image at the near point (25 cm): the eyepiece gives me=6, so the objective must give mo=5. This places the object 1.5 cm from the objective and separates the two lenses by about 11.67 cm.
Given
fo=1.25 cm, fe=5 cm, desired magnifying power M=30, near point D=25 cm.
Step 1 — Eyepiece magnification (image at near point)
me=1+feD=1+525=6
Step 2 — Objective magnification
Since M=mo×me,
mo=meM=630=5
Step 3 — Object position at the objective
The objective forms a real, inverted image, so ∣vo∣=5∣uo∣. Using vo1−uo1=fo1 with uo<0, vo>0:
5∣uo∣1+∣uo∣1=1.251⇒5∣uo∣6=1.251
∣uo∣=56×1.25=1.5 cm,vo=5×1.5=7.5 cm
The object sits 1.5 cm from the objective, just beyond its focus fo=1.25 cm.
Step 4 — Eyepiece object distance …
Method: Two-Lens Ray Diagram Approach for Compound Microscope Setup
This method uses the magnification formula for a compound microscope to determine the required tube length and lens positions.
Step 1: Recall the magnification formula
For a compound microscope in normal adjustment (final image at infinity), the total angular magnification is:
M=mo×me=(−foL)×(feD)
Where:
- M = total angular magnification (magnifying power)
- mo = linear magnification of objective
- me = angular magnification of eyepiece
- L = tube length (distance between second focal point of objective and first focal point of eyepiece)
- fo = focal length of objective = 1.25 cm
- fe = focal length of eyepiece = 5 cm
- D = least distance of distinct vision = 25 cm (standard value)
Step 2: Substitute known values
Given M=30X:
30=(−1.25L)×(525)
30=(−1.25L)×5
Step 3: Solve for tube length L
−1.25L=530=6
L=−6×1.25=−7.5 cm
The negative sign indicates the image formed by the objective is real and inverted (as expected). The magnitude gives:
Tube length L=7.5 cm
Step 4: Determine lens positions
The tube length L is the distance between: …
Here are the common mistakes students make when solving this compound microscope problem, along with how to avoid each.
Mistake 1: Confusing Magnification for the Final Image at Infinity vs. at Near Point
The error:
Students often blindly use the formula for angular magnification when the final image is at infinity (M=foL⋅feD) without checking the problem’s condition. Here, the desired magnification is 30X, but using the infinity formula gives a different tube length.
How to avoid:
Always check which case is implied. In most exam problems, unless stated otherwise, the final image is formed at the near point (25 cm). The correct formula for that case is:
M=foL(1+feD)
where D=25 cm (least distance of distinct vision).
For this problem, using fo=1.25 cm, fe=5 cm, and M=30, you solve for L:
30=1.25L(1+525)=1.25L×6
⇒L=630×1.25=6.25 cm
So the tube length is 6.25 cm.
Mistake 2: Forgetting to Add the Eyepiece Focal Length to Get Total Microscope Length
The error:
Students stop after finding L (distance between the second focal point of the objective and the first focal point of the eyepiece) and report that as the total length of the microscope.
How to avoid:
The total length of the microscope is the distance between the objective and the eyepiece. This is:
Total length=fo+L+fe
For this problem:
Total length=1.25+6.25+5=12.5 cm
Always draw a quick ray diagram to remind yourself: the objective’s second focal point and the eyepiece’s first focal point coincide — so the physical separation includes both focal lengths.
Mistake 3: Using the Wrong Sign Convention for Lens Formula
The error:
When verifying the image distances, students plug values into the lens formula without consistent sign convention, leading to negative distances that confuse them.
How to avoid:
Use the Cartesian sign convention (distances measured from the optical centre, positive in the direction of incident light). For the objective:
- uo is negative (object is real, on left)
- fo is positive (convex lens)
- vo is positive (real image on right)
For the eyepiece:
- ue is negative (object is real, on left)
- fe is positive
- ve is negative (final virtual image on left)
This consistency prevents sign errors.
--- …
- COMEDK 2026Set 2026-A1 markMCQQ.The distance between the objective and eye piece of astronomical telescope in normal adjustment is 27 cm and its magnifying power is 8 . What is the focal length of the eye piece? (A) 12 cm (B) 3 cm (C) 6 cm (D) 24 cm
›Reveal solutionSolution
[!TLDR]
From fo+fe=27 and fo/fe=8, the eyepiece focal length is 3 cm — option (B).
Concept
For an astronomical telescope in normal adjustment, the objective and eyepiece are separated by L=fo+fe, and the magnifying power is m=fo/fe (CBSE/NCERT Class 12 Ray Optics).
Solution
Given L=fo+fe=27 cm and m=fefo=8, so fo=8fe. Substituting:
8fe+fe=27 ⇒ 9fe=27 ⇒ fe=3 cm. …
- COMEDK 2026Set 2026-M1 markMCQQ.An object is placed at an unknown distance from a convex objective lens of focal length 5 cm . The objective lens forms a real image which acts as an object for a convex eyepiece of focal length 6.25 cm . The distance between the objective and eyepiece is 20 cm . The microscope is adjusted so that the final image is formed at the least distance of distinct vision (25 cm). Which of the following is correct? A. Object distance =7.5 cm; Total magnification =10 B. Object distance = 10 cm ; Total magnification = 20 C. Object distance =5 cm; Total magnification =20 D. Object distance = 2.5 cm ; Total magnification = 10 (A) C (B) D (C) B (D) A
›Reveal solutionSolution
Working back from the eyepiece: object distance for the objective is 7.5 cm and the total magnification is 10 — matching the choice "Object distance =7.5 cm; Total magnification =10", listed as option (D).
Step 1 — Eyepiece (final image at D=25 cm).
The final virtual image is on the same side as the object, so ve=−25 cm, fe=6.25 cm.
ve1−ue1=fe1 ⇒ −251−ue1=6.251
ue1=−0.04−0.16=−0.20 ⇒ ue=−5 cm
So the intermediate image sits 5 cm in front of the eyepiece.
Step 2 — Locate the intermediate image relative to the objective.
The lenses are 20 cm apart, so the objective's real image is at
vo=20−5=15 cm.
Step 3 — Objective (find the object distance). …
- COMEDK 2025Set 2025-E1 markMCQQ.In the normal adjustment of an astronomical telescope, the objective and eyepiece are 36 cm apart. If the magnifying power of the telescope is 8 , find the focal lengths of the objective and eyepiece. (A) FO=28 cm,Fee=7 cm (B) F0=28 cm, Fe=4 cm (C) Fo=32 cm,Fee=4 cm (D) F0=4 cm, Fe=32 cm
›Reveal solutionSolution
For an astronomical telescope in normal adjustment, the tube length equals the sum of the focal lengths, and the magnifying power equals the ratio of the objective focal length to the eyepiece focal length. Solving these two equations gives fo=32 cm and fe=4 cm, which corresponds to option (C).
The key idea is that in normal adjustment, the telescope is set so that the final image is at infinity. This means the eyepiece is positioned so that the intermediate image formed by the objective lies exactly at the focal point of the eyepiece. Consequently, the distance between the objective and the eyepiece (the tube length) is simply the sum of their focal lengths:
L=fo+fe.
The magnifying power M of an astronomical telescope in normal adjustment is defined as the ratio of the angle subtended by the image to the angle subtended by the object, and it simplifies to
M=fefo.
We are given L=36 cm and M=8. So we have two equations in two unknowns — a straightforward system.
- Set up the equations From the magnifying power:
fefo=8⇒fo=8fe.
From the tube length:
fo+fe=36.
- Substitute and solve Replace fo in the second equation:
8fe+fe=36⇒9fe=36⇒fe=4 cm.
Then
fo=8×4=32 cm.
- Check against the options The pair (fo=32 cm,fe=4 cm) matches option (C). …
- COMEDK 2024Set 2024-E1 markMCQQ.A telescope has an objective of focal length 60 cm and eyepiece of focal length 5 cm. The telescope is focussed for least distance of distinct vision 300 cm away from the object. The magnification produced by the telescope at least distance of distinct vision is (A) +1.5 (B) +2 (C) −1.5 (D) −2
›Reveal solutionSolution
The objective forms a real image (m1=−41); the eyepiece throws the final image to the near point (m2=+6), giving a net magnification M=−1.5.
The telescope is used on a near object: the object sits 300cm from the objective, and the final image is formed at the least distance of distinct vision, D=25cm.
Objective (fo=60cm, u1=−300cm):
v11=fo1+u11=601−3001=3004⇒v1=+75cm
m1=u1v1=−30075=−41
Eyepiece (fe=5cm, final image v2=−25cm at the near point): …
- COMEDK 2024Set 2024-M1 markMCQQ.In the normal adjustment of an astronomical telescope, the objective and eyepiece are 32 cm apart. If the magnifying power of the telescope is 7, find the focal lengths of the objective and eyepiece. (A) fo=7 cm and fe=28 cm (B) fo=28 cm and fe=7 cm (C) fe=28 cm and fo=4 cm (D) fo=28 cm and fe=4 cm
›Reveal solutionSolution
In normal adjustment, the telescope length equals the sum of the focal lengths, and the magnifying power equals the ratio of the objective focal length to the eyepiece focal length. Solving these two equations gives fo=28 cm and fe=4 cm, so the correct option is (D).
Concept & Intuition
An astronomical telescope in normal adjustment means the final image is formed at infinity — the eyepiece is set so that the light rays emerging from it are parallel. This happens when the image formed by the objective lies exactly at the first focal point of the eyepiece. Consequently, the distance between the two lenses (the tube length) is simply the sum of their focal lengths:
L=fo+fe
The magnifying power (angular magnification) in this setting is given by the ratio of the objective’s focal length to the eyepiece’s focal length:
M=fefo
We are given L=32 cm and M=7. That gives us two equations in two unknowns — straightforward algebra.
Step-by-step solution
- Write the two conditions From normal adjustment:
fo+fe=32(1)
From magnifying power:
fefo=7(2)
- Express one variable in terms of the other From (2):
fo=7fe
- Substitute into the length equation
- COMEDK 2023Set 2023-E1 markMCQQ.A person has a normal near point 25 cm. What is the magnifying power of the simple microscope he used, if the focal length of the convex lens used is 10 cm and the final image is formed at the least distance of distinct vision? (A) 7 (B) 3.5 (C) 25 (D) 2.5
›Reveal solutionSolution
(The value D/f = 2.5 is the magnifying power for the image at INFINITY (relaxed eye), which is option (D) - not what is asked here.)
Concept: simple microscope (magnifying glass). When the final image is formed at the least distance of distinct vision D (image at the near point), the magnifying power is
M = 1 + D/f.
Given D = 25 cm, f = 10 cm:
M = 1 + 25/10 = 1 + 2.5 = 3.5. …
- KCET 2022Set B-31 markMCQQ.A convex lens of focal length ‘f’ is placed somewhere in between an object and a screen, the distance between the object and the screen is ‘x’. If the numerical value of the magnification produced by the lens is ‘m’, then the focal length of the lens is (A) m(m+1)2x (B) m(m−1)2x (C) (m+1)2mx (D) (m−1)2mx
›Reveal solutionSolution
Split the fixed object–screen distance x into u and v using the magnification ratio, then feed both into f=u+vuv.
1. Set up the geometry
The lens sits between the object and the screen, and it forms a real image on the screen. Working with numerical (unsigned) distances:
- object distance =u
- image distance =v
- they must add up to the object–screen separation:
u+v=x(1)
2. Use the magnification
For a thin lens the numerical magnification of a real image is
m=uv⟹v=mu(2)
3. Solve for u and v
Substituting (2) into (1):
u+mu=x⟹u(1+m)=x⟹u=m+1x
v=mu=m+1mx
4. Apply the lens formula
In magnitudes, the thin-lens relation for a real object and real image is
f1=u1+v1=uvu+v⟹f=u+vuv
Now substitute, noting the denominator u+v is just x:
f=x(m+1x)(m+1mx)=x(m+1)2mx2
∴f=(m+1)2mx
5. Sanity checks …
- COMEDK 2021Set 20211 markMCQQ.The magnifying power of a telescope is 9. When it is adjusted for parallel rays, the distance between the objective and eyepiece is 20 cm. The focal length of lenses are (A) 10 cm, 10 cm (B) 15 cm, 5 cm (C) 18 cm, 2 cm (D) 11 cm, 9 cm
›Reveal solutionSolution
Focal lengths: 18 cm (objective) and 2 cm (eyepiece).
Concept: astronomical telescope in normal adjustment (parallel rays / relaxed eye).
Magnifying power m = f_o/f_e = 9
Tube length L = f_o + f_e = 20 cm
Substituting f_o = 9 f_e: …
- KCET 2020Set A-11 markMCQQ.The following figure shows a beam of light converging at point P. When a concave lens of focal length 16 cm is introduced in the path of the beam at a place shown by dotted line such that OP becomes the axis of the lens, the beam converges at a distance x from the lens. The value of x will be equal to
(A) 12 cm (B) 24 cm (C) 36 cm (D) 48 cm
›Reveal solutionSolution
A beam already converging towards a point beyond the lens means that point is a virtual object (u positive); apply v1−u1=f1 with u=+12 cm, f=−16 cm.
Step 1 — Read the geometry
From the figure: light converges towards P, and the concave lens is inserted at the dotted line through O, with OP=12 cm measured along the axis, on the far (outgoing) side of the lens.
Step 2 — Identify the virtual object
If the lens were absent, the rays would meet at P. Because the rays are already converging when they strike the lens, they never actually diverge from a real object point. The point P — where they would have met — acts as a virtual object.
In the Cartesian sign convention (light travelling left → right, distances measured from the optical centre, rightward positive):
u=+12 cm(virtual object, on the outgoing side)
f=−16 cm(concave / diverging lens)
The positive u is the whole trick of the question — a real object would give a negative u.
Step 3 — Apply the lens formula
v1−u1=f1 …
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