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Q.A true breeding homozygous pea plant with round seeds and yellow cotyledons is crossed with homozygous pea plant with wrinkled seeds and green cotyledons.

(a) Work out the cross to show the phenotype and genotype ratio of F1 and F2 generations.
(b) State the Mendel's generalisation that can be derived from above cross.
Punjab PsebPSEB Punjab Class 12 Board 2019Subjective· 4mImportance★★★★★
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A dihybrid cross of RRYY x rryy gives an all-RrYy F1, and F2 shows the classic 9:3:3:1 ratio, proving that the two gene pairs assort independently of each other.

(a) The cross:

Round seed shape (R, dominant) vs wrinkled (r, recessive); Yellow cotyledon colour (Y, dominant) vs green (y, recessive).

Parents: RRYY (round, yellow) x rryy (wrinkled, green)

Gametes: RY (from RRYY) and ry (from rryy)

F1 generation: all offspring are RrYy -- Round, Yellow (since R and Y are both dominant, they mask r and y).

When F1 (RrYy) is self-pollinated, each parent produces 4 types of gametes in equal proportion: RY, Ry, rY, ry. A 4x4 Punnett square gives 16 combinations in F2:

  • 9 parts R_Y_ -> Round, Yellow
  • 3 parts R_yy -> Round, Green
  • 3 parts rrY_ -> Wrinkled, Yellow
  • 1 part rryy -> Wrinkled, Green

So the F2 phenotypic ratio is 9 : 3 : 3 : 1 (Round Yellow : Round Green : Wrinkled Yellow : Wrinkled Green), and the genotypic ratio is the standard 1:2:1:2:4:2:1:2:1 (9 genotype classes).

(b) Mendel's generalisation: …

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