Q.State and explain Mendel's Law of Independent Assortment with reference to a dihybrid cross involving pea plant. Consider the traits of seed shape (round vs. wrinkled) and seed colour (yellow vs. green). OR Given below is a diagram of the Lac operon in the presence of an inducer. Carefully observe the diagram and answer the following questions :
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Start your 14-day free trial to unlock the full solution →Mendel's Law of Independent Assortment states that when two pairs of traits are considered together, each pair segregates into gametes independently of the other pair, producing all possible allele combinations in a 9:3:3:1 ratio in the F2 generation.
Mendel's Law of Independent Assortment (Law of Independent Segregation of Genes): when a dihybrid (an organism heterozygous for two different genes) forms gametes, the alleles of one gene segregate into gametes independently of how the alleles of the other gene segregate, so gametes are produced with all possible combinations of the two traits in equal proportion.
Demonstration using a pea dihybrid cross (seed shape × seed colour):
Let R = Round (dominant), r = wrinkled (recessive); Y = Yellow (dominant), y = green (recessive).
- Parental (P) cross: RRYY (round, yellow) × rryy (wrinkled, green)
- F1 generation: All offspring are RrYy — round and yellow (since Round and Yellow are dominant), showing the two traits are together inherited but each is still a separate hybrid pair internally.
- F1 self-pollination: RrYy × RrYy. Each F1 plant produces four types of gametes in equal proportion — RY, Ry, rY, ry — because the R/r pair assorts independently of the Y/y pair during gamete formation (this is the crux of the law).
- F2 generation (using a Punnett square of the 4×4 gamete combinations): phenotypes appear in the ratio:
- 9 Round Yellow
- 3 Round green
- 3 wrinkled Yellow
- 1 wrinkled green …
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