Q.What are the factors affecting the rate of enzyme reaction?
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Start your 14-day free trial to unlock the full solution →Step 1. Temperature. Heating increases molecular motion, so enzyme and substrate molecules collide more often and more forcefully, raising the reaction rate up to an optimum temperature; beyond that point the enzyme's protein structure begins to denature and activity falls sharply.
Step 2. pH. Every enzyme has an optimum pH at which its active-site shape is ideal for binding substrate; moving away from this optimum alters the ionisation of the active site's amino acid residues, changing its shape, and extreme pH denatures the enzyme entirely (e.g., pepsin works best around pH 2, while catalase prefers a near-neutral pH). …
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