Q.Give an account on Cryopreservation.

Step 1. Cryopreservation, also called cryo-conservation, is the process of preserving protoplasts, cells, tissues, organelles, organs, extracellular matrix, enzymes or other biological material by cooling it to an extremely low temperature of about -196 degC, using liquid nitrogen.

Step 2. At this temperature, all enzymatic and chemical activity within the material stops completely, which halts ageing and decay and leaves the material preserved in a dormant, stable state.

Step 3. Because ice-crystal formation during freezing and thawing can damage cell membranes, protective agents called cryoprotectants - dimethyl sulphoxide, glycerol or sucrose are named examples - are added to the material before cooling to shield it from this stress. …

Cover the definition, the cooling medium and temperature, the role of cryoprote …

• Omitting the role of cryoprotectants in protecting against freezing damage. …