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NCERT Exemplar · Q2

Q.A nucleoside differs from a nucleotide. It lacks the:

(a) base
(b) sugar
(c) phosphate group
(d) hydroxyl group
Uttarakhand UbseMCQ· 1mImportance★★★★★
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✓ Free question

A nucleoside is a base + sugar; a nucleotide is a base + sugar + phosphate. The key missing piece in a nucleoside is the phosphate group.

The question asks you to spot the difference between a nucleoside and a nucleotide. This is a fundamental distinction in molecular biology, and it comes down to one simple structural addition.

Think of a nucleoside as the core building block. It consists of a nitrogenous base (adenine, guanine, cytosine, thymine, or uracil) attached to a sugar molecule (ribose in RNA, deoxyribose in DNA). That's it — just two components linked together.

A nucleotide, on the other hand, is a nucleoside that has been further modified. A phosphate group is attached to the sugar's 5' carbon. So a nucleotide is always a nucleoside plus one or more phosphate groups. This phosphate is what gives nucleotides their acidic nature and their ability to link together to form nucleic acid chains.

Note

The NCERT textbook makes this distinction very clearly: "A nucleotide consists of three components — a nitrogenous base, a pentose sugar and a phosphate group." A nucleoside, by contrast, is defined as "a nitrogenous base linked to a pentose sugar only."

Now look at the options given:

  • (A) base — Both nucleosides and nucleotides have a nitrogenous base. So this is not the missing part.
  • (B) sugar — Both have a pentose sugar (ribose or deoxyribose). So this is also present in both.
  • (C) phosphate group — This is present in a nucleotide but absent in a nucleoside. This is the correct answer.
  • (D) hydroxyl group — Both have hydroxyl groups on the sugar (though deoxyribose lacks one at the 2' position). This is not the distinguishing feature.
Important

A common confusion is thinking that a nucleoside lacks the sugar's hydroxyl group. That is wrong. The hydroxyl groups are part of the sugar itself. The only component that a nucleoside lacks, compared to a nucleotide, is the phosphate group.

So when you see a diagram in your NCERT textbook showing a nucleoside, you'll notice it has just the base and the sugar ring. The nucleotide diagram will show the same structure but with a phosphate group attached to the 5' carbon of the sugar.

✓Final answer

In short, a nucleoside differs from a nucleotide because it lacks the phosphate group. The correct option is (C).

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