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Long Answer Questions · Q28

Q.Trace, step by step, the digestion of carbohydrates, proteins and fats as food passes through the mouth, stomach and small intestine, naming the enzymes involved and their end products at each stage.

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Carbohydrate digestion begins uniquely in the mouth, where salivary amylase (ptyalin) partially breaks cooked starch down to maltose; this halts in the acidic stomach and resumes in the duodenum, where pancreatic amylase continues the breakdown of remaining starch to maltose, which is finally split by the brush-border disaccharidases maltase, sucrase and lactase into the absorbable monosaccharides glucose, fructose and galactose. Protein digestion begins only in the stomach, where pepsin (activated from pepsinogen by HCl) breaks dietary protein into proteoses and peptones; in the duodenum, the pancreatic proteases trypsin and chymotrypsin (each activated from an inactive zymogen -- trypsinogen by enterokinase, chymotrypsinogen by trypsin) reduce these to smaller peptides, and carboxypeptidase removes terminal amino acids, with the final splitting to free amino acids done by brush-border peptidases. Fat digestion is essentially confined to the duodenum: dietary fat is first emulsified by bile salts into small droplets (a physical, non-enzymatic step that increases surface area), after which pancreatic lipase hydrolyses the triglycerides at each droplet's surface into free fatty acids and monoglycerides, which bile salts then package into micelles for delivery to the intestinal brush border. In every case, the final digestion products -- monosaccharides, amino acids, and fatty acids/monoglycerides -- are the only forms in which carbohydrate, protein and fat can actually be absorbed across the intestinal epithelium.

[!ANSWER]

Carbohydrate: starch -> (salivary + pancreatic amylase) -> maltose -> (brush-border disaccharidases) -> monosaccharides. Protein: protein -> (pepsin) -> proteoses/peptones -> (trypsin/chymotrypsin/carboxypeptidase) -> peptides -> (brush-border peptidases) -> amino acids. Fat: triglyceride -> (bile emulsification, then pancreatic lipase) -> fatty acids + monoglycerides.

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