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Long Answer Questions · Q32

Q.Discuss the respiratory quotient (RQ) -- its definition and formula, its value for carbohydrates, fats, proteins and organic acids, with the chemical reasoning behind each value, and its significance in determining the nature of the substrate being respired by a tissue.

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The respiratory quotient (RQ) is defined as the ratio of the volume of carbon dioxide evolved by a respiring tissue to the volume of oxygen it consumes, over the same time period and under the same conditions: RQ = CO₂ evolved / O₂ consumed.

For carbohydrates such as glucose, complete oxidation follows C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O, consuming 6 moles of O2 and releasing 6 moles of CO2, giving RQ = 1; this is because carbohydrates already contain hydrogen and oxygen in the same ratio as water, so no extra oxygen beyond what balances the carbon released as CO2 is needed.

For fats, the RQ works out distinctly less than 1, commonly cited as about 0.7 for a typical fat such as tripalmitin. Fats are chemically more reduced than carbohydrates -- a far higher proportion of carbon and hydrogen and comparatively little oxygen -- so completely oxidising a fat molecule requires substantially more external oxygen relative to the CO2 it eventually releases, pulling the ratio below 1.

For proteins, the RQ takes an intermediate value, commonly cited as about 0.9, reflecting both an intermediate oxygen content and the fact that proteins are not, strictly, fully oxidised by respiration -- the nitrogenous amino group of each amino acid is removed by deamination before the remaining carbon skeleton enters the respiratory pathway. …

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