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NCERT Exemplar · Q24

Q.Nucleic acids exhibit secondary structure, justify with example.

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Nucleic acids do exhibit secondary structure, and DNA's double helix is the standard example: two antiparallel polynucleotide strands coiled around a common axis, held together by hydrogen bonds between complementary base pairs (A-T, G-C).

A nucleic acid, whether DNA or RNA, is built as a polynucleotide — a long chain formed by linking nucleotides one after another through phosphodiester bonds. Each nucleotide is itself a combination of a nitrogenous base (a purine such as adenine or guanine, or a pyrimidine such as cytosine, thymine or uracil), a pentose sugar (deoxyribose for DNA, ribose for RNA), and a phosphate group. Taken purely as this linear sequence of bases, a nucleic acid has only a primary structure.

DNA, however, does not exist in the cell as a bare, unstructured single strand. Watson and Crick proposed — and it has since been confirmed — that DNA occurs as a double helix: two polynucleotide chains wound around a common central axis, like a twisted ladder. This coiled, two-stranded arrangement, over and above the plain linear sequence of nucleotides in either strand, is what constitutes the secondary structure of DNA.

Two further structural facts justify calling this a genuine, specific secondary structure rather than a vague coil:

  • The strands are antiparallel. The two polynucleotide chains run in opposite chemical directions — the 5'-phosphate end of one strand is aligned against the 3'-hydroxyl end of the other. …

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