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Q.(a) How would you find out whether a given tall garden pea plant is homozygous or heterozygous ? Substantiate your answer with the help of Punnett squares.

(b) Given below are the F2-phenotypic ratios of two independently carried monohybrid crosses :
(i) 1 : 2 : 1
(ii) 3 : 1 Mention what does each ratio suggest.
(OR)
(a) Why did Hershey and Chase use radioactive 32P and 35S in their experiments ? Explain.
(b) Following the experiments conducted by them, write what conclusion did they arrive at and how.
CBSECBSE Class XII Board 2019Subjective· 5mImportance★★★★★
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Part (a): A test cross (unknown tall × dwarf tt) tells homozygous from heterozygous — all-tall offspring means TT, a 1:1 ratio means Tt; F2 of 1:2:1 = incomplete dominance/codominance, 3:1 = complete dominance.

Part (b): Hershey and Chase labelled DNA with 32P and protein with 35S; only DNA entered the bacterial cell, proving DNA is the genetic material.

Part (a)

A tall pea plant can be homozygous (TT) or heterozygous (Tt) — both look tall because T is dominant. To distinguish them we use a test cross: cross the unknown with a homozygous recessive dwarf (tt), which can only donate t gametes.

If the tall plant is TT:

×TT
tTtTt
tTtTt

All offspring are Tt → all tall.

If the tall plant is Tt:

×Tt
tTttt
tTttt

Offspring are Tt : tt = 1:1 → half tall, half dwarf.

F2 phenotypic ratios:

  • (i) 1 : 2 : 1 — the phenotypic ratio equals the genotypic ratio, so the heterozygote has its own distinct phenotype. This indicates incomplete dominance (e.g. red × white Antirrhinum → pink) or codominance (e.g. IAIB AB blood group). …

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