Q.Identify true statement regarding light reaction of photosynthesis?
a. Splitting of water molecule is associate with PS I.
b. PS I and PS II involved in the formation of NADPH+H+.
c. The reaction center of PS I is Chlorophyll a with absorption peak at 680 nm.
d. The reaction center of PS II is Chlorophyll a with absorption peak at 700 nm.

Light Reaction: The Engine That Runs on Sunlight

Imagine a solar panel. Sunlight hits it, and out comes electricity. The light reaction in photosynthesis is exactly that — a solar panel built into every green leaf. But instead of electricity, it produces two chemical fuels: ATP (the energy currency of the cell) and NADPH (a reducing agent that carries high-energy electrons). These two molecules are then used in the next phase (the Calvin cycle) to build glucose from carbon dioxide.

The key insight: the light reaction is the only part of photosynthesis that directly uses light. Everything after it runs on the ATP and NADPH it produces.

Where does it happen?

Inside the chloroplast, there are flattened, disc-like sacs called thylakoids. Their membranes are packed with pigment molecules (chlorophyll a, chlorophyll b, carotenoids) and protein complexes. This is the stage. The space inside the thylakoid is the lumen; the fluid outside is the stroma.

What actually happens? (The precise statement)

When a photon of light strikes a chlorophyll molecule, an electron gets excited — it jumps to a higher energy level. That excited electron is then passed through a chain of carriers embedded in the thylakoid membrane, like a bucket brigade. As it moves, its energy is used to pump protons ($H^+$) from the stroma into the thylakoid lumen, building up a concentration gradient. That gradient drives ATP synthesis (via ATP synthase). Meanwhile, the electron eventually ends up reducing NADP$^+$ to NADPH.

But here's the catch: the chlorophyll that lost an electron needs to replace it. That electron comes from water ($H_2O$). Splitting water releases oxygen gas ($O_2$) as a byproduct — that's the oxygen we breathe.

The two photosystems: a closer look

There are two distinct pigment systems, Photosystem II (PSII) and Photosystem I (PSI), named in the order they were discovered, not the order they work. Light hits both.

1. PSII absorbs light, excites an electron, and passes it to an electron acceptor. The electron hole in PSII is filled by splitting water. This releases $O_2$ and protons into the lumen.
2. The electron travels down an electron transport chain (cytochrome $b_6f$ complex), pumping protons along the way.
3. The electron reaches PSI, which also absorbs light and re-excites the electron to an even higher energy level.
4. That high-energy electron is finally used to reduce NADP$^+$ to NADPH.

The proton gradient built up during step 2 drives ATP synthase to make ATP. This entire flow is called non-cyclic photophosphorylation — the most common pathway.

Why does water split? …