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Evaluation · Q39

Q.Write the steps involved in molecular mechanism of DNA recombination with diagram.

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Step 1. Two homologous DNA duplexes, each already duplicated, first align closely side by side.

Step 2. An endonuclease nicks one strand of each duplex at the identical position.

Step 3. The cut strands cross over and anneal to the homologous strand on the partner duplex, forming a four-stranded Holliday junction.

Step 4. The junction slides along the DNA (branch migration), extending a heteroduplex region. …

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