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Question 39 of 56

Q.What is DNA library ?

Puducherry TnboardTamil Nadu HSC (DGE) Board 2019Subjective· 3mImportance★★★★★
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A DNA library is an organised, clonable collection of an organism's DNA fragments held in vectors, allowing any gene to be recovered when needed.

To build a DNA library, an organism's total genomic DNA (or its mRNA, converted to complementary DNA) is cut into fragments, each of which is inserted into a cloning vector (like a plasmid or bacteriophage) and introduced into host bacterial or yeast cells, where it is replicated and maintained. A genomic library contains fragments representing the organism's entire DNA content (including non-coding regions), while a cDNA library contains only the sequences that are actively expressed as mRNA in a particular cell type at a particular time — making cDNA lib …

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