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Post-transcriptional Processing · Q25

Q.Why does bacterial mRNA require little or no post-transcriptional processing, while eukaryotic hnRNA requires extensive processing before it can be translated?

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✓ Free question

Step 1. Bacteria have no nuclear membrane separating their DNA from their ribosomes, so a newly transcribed mRNA is generally translated at the ribosome directly and immediately, often even while transcription of that same molecule is still ongoing.

Step 2. Because translation begins essentially as soon as (or before) transcription finishes, there is no time or physical need for extensive further modification of bacterial mRNA — it is used essentially as-is.

Step 3. Eukaryotic cells DO have a nuclear membrane, physically separating the nucleus (where transcription occurs) from the cytoplasm (where translation occurs at the ribosome).

Step 4. Because eukaryotic RNA must be exported out of the nucleus before it can be translated, and because the primary transcript (hnRNA) includes non-coding introns, it must first undergo processing — splicing, capping and tailing — before it is a mature, translatable mRNA ready for export.

✓Final answer

The absence of a nuclear membrane lets bacterial mRNA be translated immediately with little processing; eukaryotic hnRNA is made in a separate nuclear compartment and must be processed before export and translation.

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