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Evaluation · Q11

Q.What is the name of the process given below? Write its 4 types. [Figure: a flow diagram of the basic steps in plant tissue culture technology - a tissue sample from a bud, stem, root or leaf is taken as the explant; it is inoculated and becomes undifferentiated; a callus is formed; the callus is separated and single cells are cultured; further culturing regenerates a new plantlet.]

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Step 1. The diagram traces a tissue sample (explant) taken from a bud, stem, root or leaf through an undifferentiated stage, callus formation, separation and single-cell culturing, and further culturing to a regenerated new plantlet - this is the general sequence followed by Plant Tissue Culture (PTC).

Step 2. PTC is classified into four types based on which part of the plant is used as the explant.

Step 3. Organ culture uses whole organs - embryos, anthers, ovaries, roots or shoots.

Step 4. Meristem culture uses the plant's actively dividing meristematic tissue, most often the shoot-tip apical meristem. …

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