Q.Write the various steps involved in cell suspension culture.

Cell suspension culture is the growth of single cells or small aggregates of cells directly in a liquid medium, prepared by transferring a portion of an existing callus into liquid medium and agitating it on a rotary shaker, which both separates cells from the callus and keeps them suspended and aerated. Cell suspension culture is the platform of choice for industrial-scale production of plant secondary metabolites - chemical compounds such as alkaloids, flavonoids, terpenoids, phenolic compounds and recombinant proteins that a plant does not need for its own growth but produces as byproducts of its normal metabolism. A classic example is the production of indole alkaloids from Catharanthus roseus cell culture. At commercial scale, production is carried out in bio-reactors, with techniques such as biotransformation, elicitation and immobilization used to imp …