Q.Name the RBC and WBC diluting fluids used in blood cell counting.

Blood cell counting under a haemocytometer requires dilution with a specific fluid for each cell type: Hayem's fluid for RBCs and Turk's fluid for WBCs.

Because whole blood contains far too many cells to count accurately under a microscope, it is first diluted with a specific diluting fluid before being loaded onto a haemocytometer (counting chamber) for total blood cell counts. For counting red blood cells (RBC count), the blood sample is diluted using Hayem's fluid, an isotonic solution (containing sodium sulphate, sodium chloride, and mercuric chloride) that dilutes the blood without destroying or altering the red blood cells, allowing them to be counted intact under the microscope. For counting white blood cells (WBC count / total leucocyte count), the blood sample is instead diluted using Turk's fluid, which contains a weak acid (acetic acid) that lyses (destroys) the red blood cells present in the sample while leaving the white bl …