Q.Diluting fluid used for total RBC count is:

A haemocytometer is a thick, precisely ruled glass slide used to count blood cells under the microscope. Its central counting chamber follows the improved Neubauer pattern: a 3x3 mm primary square divided into nine 1x1 mm secondary squares - the four corner squares (each further split into 16 tertiary squares) used for WBC counting, and the central secondary square (split into 25 still-smaller tertiary squares) used for RBC and platelet counting. Blood is diluted before counting to keep cells intact: Hayem's fluid (isotonic, preventing haemolysis) is used for RBC counts (diluted 1:200, counted under the 45x objective), while Turk's solution (glacial acetic acid plus gentian violet) is used for WBC counts (diluted 1:20, counted under the 10x objective) - the acetic acid lyse …