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Evaluation · Q33

Q."Tissue culture is the best method for propagating rare and endangered plant species" - Discuss.

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Step 1. Tissue culture rests on the principle of totipotency - a single plant cell, given a suitable medium and controlled conditions, can be induced to divide and regenerate an entire new plant, as F.C. Steward first demonstrated with carrot phloem cells.

Step 2. This means a rare or endangered plant can be multiplied starting from just a tiny amount of source tissue, without needing to harvest large quantities of plant material that a scarce population may not be able to spare.

Step 3. Tissue culture (micropropagation) can be carried out in any season and does not depend on the plant setting viable seed - a real advantage for endangered species that may produce little or no fertile seed, or whose seeds are difficult to germinate. …

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