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Question 41 of 74

Q.What are the methods of diagnosis of AIDS ?

Tamil Nadu DgeTamil Nadu HSC (DGE) Board 2017Subjective· 3mImportance★★★★★
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AIDS is diagnosed by detecting HIV antibodies with ELISA, confirmed by Western blot, and monitored using PCR and CD4+ T-cell counts.

Methods of diagnosis of AIDS:

  • ELISA (Enzyme-Linked Immunosorbent Assay): the standard screening test that detects antibodies produced by the body against HIV in the blood serum. It is widely used because it is quick and inexpensive, but a positive result needs confirmation.
  • Western blot test: a more specific confirmatory test performed after a positive ELISA result, which detects antibodies against specific HIV proteins, reducing the chance of a false positive.
  • PCR (Polymerase Chain Reaction): detects the viral RNA/DNA directly, allowing diagnosis even before antibodies develop (the 'window period'), and is useful for early detection and for testing infants born to HIV-positive mothers. …

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