Q.What are 'primers' in a PCR reaction? Explain why two different primers are needed for amplifying a specific segment of double-stranded DNA.

PCR is a common laboratory technique used to make millions of copies of a chosen region of DNA. In recombinant DNA technology, it serves as the alternative route to vector-based cloning wherever no vector is actually used: instead of inserting the gene of interest into a self-replicating vector and propagating it inside a host cell, PCR directly amplifies that gene of interest in vitro, afte …