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Q.Highly conserved proteins such as Haemoglobin and Cytochrome-C provide the best biochemical evidences to trace evolutionary relationships between different groups. Cytochrome-C is formed of 104 amino acids. Cytochrome-C is the respiratory pigment present in all eukaryotic cells. It has evolved at a constant rate during evolution. In chimpanzees and humans, Cytochrome-C genes are identical. The given data shows the evolution of the Cytochrome-C gene in different mammals from kangaroos, cows, rodents to humans : [Table — Groups / Nucleotide substitution in the gene of Cytochrome-C / Millions of years ago: Human/Kangaroo — 100 — 125 mya Human/Cow — 75 — 120 mya Human/Rodent — 60 — 75 mya]

(a) Select the correct option for the time of separation of two groups and the number of nucleotide substitutions in the gene of Cytochrome-C : [Options / Time of separation of two groups during evolution / Number of nucleotide substitutions:
(i) Lesser / Greater
(ii) Greater / Lesser
(iii) Greater / Greater]
(b) What do you infer about the type of evolution (convergent or divergent) for the given pair of groups and why ?
(i) Human and Kangaroo
(ii) Human and Rodent
(c)
(i) Define convergent evolution. OR
(c)
(ii) Define divergent evolution.
CBSECBSE Class XII Board 2025Subjective· 4mImportance★★★★★
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Part (a): more time since divergence means more Cytochrome-C substitutions, so option (iii) Greater/Greater is correct; both human–kangaroo and human–rodent are divergent evolution; convergent evolution = independent origin of similar traits in unrelated lineages.

Part (c): divergent evolution = accumulation of differences in descendants of a common ancestor (homologous structures).

Highly conserved proteins like Cytochrome-C evolve at a nearly constant rate, so the count of nucleotide substitutions in the gene between two groups measures how long ago their lineages split — a molecular clock.

Part (a)

  1. Choosing the correct option. Reading the table: Human/Kangaroo — 100 substitutions, separated ~125 mya (oldest split, most differences); Human/Cow — 75 substitutions, ~120 mya; Human/Rodent — 60 substitutions, ~75 mya (most recent split, fewest differences). The trend is clear and directly proportional: the greater the time of separation, the greater the number of substitutions. Option (i) Lesser/Greater and (ii) Greater/Lesser both contradict the data, so the answer is (iii) Greater / Greater.
  2. Type of evolution for the pairs. Cytochrome-C is a homologous gene present in all these mammals; differences arise as descendants of a shared ancestor accumulate mutations — the signature of divergent evolution.
  • (i) Human and Kangaroo: divergent — a common ancestor ~125 mya, since when 100 substitutions have built up.
  • (ii) Human and Rodent: divergent — a more recent common ancestor ~75 mya, with 60 substitutions accumulated. …

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