EVALUATION

This closing Evaluation set draws on essentially every subsection covered in the chapter, running from the basic vocabulary of genetic engineering (restriction enzymes, plasmids, EcoRI's recognition sequence, pBR322) through the correct sequence of steps in building a recombinant DNA molecule, palindromic restriction sites, enzyme-function matching (exonuclease, endonuclease, alkaline phosphatase, ligase), Ethidium Bromide's role in gel visualisation, Agrobacterium/Ti-plasmid biology, Southern hybridisation, antibiotic-resistance selection markers, and Bt cotton's real characteristics, before moving into longer descriptive questions on fermentation/SCP inputs, restriction-enzyme classification, gene-transfer chemicals, vector identification, blotting-technique comparison, herbicide-tolerant crops, Bt cotton's advantages and disadvantages, bioremediation, and t …