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Evaluation · Q5

Q.Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.
Choose the correct option regarding above statements
a. I & II
b. I & III
c. II & III
d. I,II & III

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Step 1. Check statement I. The chapter describes recombinant DNA technology/genetic engineering exactly this way: a stream of biotechnology dealing with the manipulation of genetic material by man, in-vitro. This statement is true.

Step 2. Check statement II. The chapter confirms pBR322 was developed by the scientists Bolivar and Rodriguez, constructed from an E. coli plasmid, and that 'p' denotes plasmid and 'BR' their names - matching statement II's claim, including the plasmid's well-documented 1977 origin. This statement is true. …

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