Q.An analysis of chromosomal DNA using the southern hybridisation technique does not use

Step 1. Confirm electrophoresis is used. The chapter's steps for Southern blotting begin with DNA being cleaved by restriction enzymes and separated by gel electrophoresis - so electrophoresis is genuinely part of the technique.

Step 2. Confirm blotting is used. The whole technique is defined by transferring (blotting) the denatured DNA from the agarose gel onto a nitrocellulose/nylon membrane by capillary action - so blotting is obviously central to it.

Step 3. Confirm autoradiography is used. The chapter states the hybridised DNA 'can be detected by autoradiography' as the final visualisation step of Southern blotting. …

Walk through each option against the actual steps of Southern blotting given in the chapter, and identify the one t …

• Assuming PCR must be involved simply because it is another well-known DNA technique in the same chapter. …