Q.A fresh section was taken by a student but he was very disappointed because there were only few green and most colourless cells. Teacher provided a pink colour solution. The section was immersed in this solution and when observed it was much clearer. What is the magic?
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Start your 14-day free trial to unlock the full solution →Step 1. Freshly cut, unstained plant sections are mostly transparent to pale, since living cell contents (cytoplasm, cell sap) and thin cellulose walls do not absorb visible light strongly — only cells with chlorophyll appear green, and the rest look colourless under the microscope.
Step 2. A pink microscopy stain used on plant sections binds preferentially to lignin, the substance that thickens and hardens the walls of xylem tracheids, vessels and fibres (and sclerenchyma generally).
Step 3. When the section is immersed in the stain, only the lignified xylem walls take up the pink colour strongly, while the thin, cellulose-only walls of parenchyma and other unlignified tissue stay largely unstained. …
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