Q.Differential count of WBCs.

A differential count reports the percentage of each type of leucocyte among 100 WBCs counted on a stained blood smear - normally about 60-70 % neutrophils, 25-30 % lymphocytes, 3-5 % monocytes, 1-3 % eosinophils and 0.5-1 % basophils. …

Suggested plan.

1. Smear - a drop of blood on a clean slide spread thinly with a second slide, air-dried and stained with a Leishman or Giemsa stain; rinse and dry.
2. Identify under the high-power objective using section 8.11 and Fig. 8.15: neutrophil (2-7 lobed nucleus, fine pale granules), eosinophil (bilobed nucleus, coarse red granules), basophil (few large dark granules, twisted nucleus), lymphocyte (small cell almost filled by a round nucleus), monocyte (largest cell, kidney-shaped nucleus).
3. Count - move across the smear in a zig-zag so no cell is counted twice, tallying each type until 100 WBCs have been counted; each tally is then the percentage. …