Concept understanding — Cyclic and Non-Cyclic Photophosphorylation
The Big Picture: Why Plants Need Two Kinds of Light Reactions
Photosynthesis has two jobs: make ATP (the energy currency) and make NADPH (the reducing power). Calvin cycle needs both — 3 ATP and 2 NADPH for every molecule of G3P it builds. But here's the problem: the light reactions don't always produce ATP and NADPH in that exact ratio. Sometimes the cell needs extra ATP without extra NADPH. That's where the two pathways come in.
Think of it like a factory. Non-cyclic photophosphorylation is the main assembly line — it produces both ATP and NADPH, plus oxygen as a byproduct. Cyclic photophosphorylation is a backup loop — it only makes ATP, no NADPH, no oxygen. The plant switches between them depending on what the Calvin cycle needs at that moment.
Non-Cyclic Photophosphorylation: The Main Line
This is the standard Z-scheme you see in every textbook. It involves both photosystems — PS II and PS I — working in series.
Step by step:
Light hits PS II (P680). An electron gets excited and is passed to a primary acceptor.
That electron is replaced by splitting water: 2H2O→4H++4e−+O2. This is the only source of oxygen in photosynthesis.
The excited electron travels down an electron transport chain (ETC) from PS II to PS I. As it moves through the cytochrome b6f complex, it pumps protons (H+) into the thylakoid lumen, building a gradient.
That proton gradient drives ATP synthase — photophosphorylation happens here. ATP is made.
The electron, now lower in energy, reaches PS I (P700). Light hits PS I, re-exciting the electron to a much higher energy level.
This high-energy electron is passed to ferredoxin (Fd) and then to NADP+ reductase, which reduces NADP+ to NADPH.
2H2O+2NADP++3ADP+3PilightO2+2NADPH+3ATP
The key point: electrons flow from water to NADP+ in a one-way path. They never return to where they started. That's why it's called non-cyclic.
Important
Non-cyclic photophosphorylation produces ATP, NADPH, and O2. It requires both PS II and PS I.
Cyclic Photophosphorylation: The Shortcut
Sometimes the Calvin cycle has enough NADPH but needs more ATP. The plant can't just make ATP without making NADPH — unless it uses a different route.
In cyclic photophosphorylation, only PS I is involved. PS II and water splitting are completely bypassed.
How it works:
Light excites PS I (P700). An electron is ejected and picked up by ferredoxin.
Instead of going to NADP+, the electron is shunted back to the cytochrome b6f complex (via plastoquinone).
It travels down the ETC again, pumping protons — generating ATP.
The electron eventually returns to PS I, filling the hole it left.
The electron goes in a circle: PS I → ferredoxin → plastoquinone → cytochrome b6f → plastocyanin → PS I. No water is split, no oxygen is released, no NADPH is made.
ADP+PilightATP(only ATP, no NADPH, no O2)
Watch out
A common mistake: thinking cyclic photophosphorylation produces NADPH. It does not. The electron never reaches NADP+ reductase — it loops back to PS I.
ATP synthesis during the light reaction of photosynthesis can proceed by two distinct routes, depending on whether one or both photosystems are involved and whether the excited electron eventually returns to its source or moves onward to reduce NADP+. …
Cyclic photophosphorylation involves only Photosystem I in a closed electron loop, producing ATP only (no NADPH, no O2).
Cyclic phosphorylation uses only PS I and yields ATP alone; non-cyclic phosphorylation uses both photosystems in series and yields ATP, NADPH and O2.
Cyclic photophosphorylation involves only Photosystem I. When PS I is excited by light, the ejected electron passes through a chain of electron carriers (such as ferredoxin and cytochrome b6f complex) and eventually returns to PS I itself, completing a closed cycle. This electron flow generates a proton gradient across the thylakoid membrane that is used to synthesise ATP, but since the electron returns to its source, no NADPH is produced and no water is split, so no oxygen is released. It typically occurs in stroma lamellae, where PS II is absent, and operates when the cell requires extra ATP relative to NADPH. …
Same / Similar Concept — real previous-year questions on the same or a closely similar concept, not this exact question.
CBSE 2021Set ANNUAL1 mark
Q.What is photo-phosphorylation?
›Reveal solutionSolution
ATP is generated in the thylakoid membrane during the light reaction, using energy from the electron transport chain set up by light absorption.
During the light reaction of photosynthesis, light energy absorbed by the photosystems drives electron flow through an electron transport chain embedded in the thylakoid membrane. This electron flow pumps protons (H+) into the thylakoid lumen, building up a proton gradient across the membrane. As these protons flow back out through the enzyme ATP synthase, the energy released is used to synthesize ATP from ADP and inorganic phosphate — this process is called photophosphorylation. It occurs in two …